Serology QUIZ & LAB Flashcards

1
Q

Which leukocyte is responsible for the synthesis and secretion of a specific antibody?
1. T lymphocyte
2. Neutrophil
3. Macrophage
4. Plasma cell

A
  1. Plasma cell
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2
Q

When evaluating an animal for active infection of a specific disease, the minimum serology requirements are:
1. the convalescent titer is 4x higher than the acute titer
2. the convalescent titer is 2x higher than the acute titer
3. the acute titer is 4x higher than the convalescent titer
4. the acute titer is 2x higher than the convalescent titer

A
  1. the convalescent titer is 4x higher than the acute titer
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3
Q

The minimum time separation between a diagnostic acute and convalescent titer is:
a. 20 days
b. 2 days
c. 2 months
d. 2 weeks

A

2 weeks

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4
Q

An antigen-antibody reaction that increases an antigen’s susceptibility to phagocytosis is:
1. complement
2. ELISA
3. colloidal gold
4. opsonization

A
  1. opsonization

This is a crucial process in the immune system that helps to identify and eliminate foreign invaders, such as bacteria and viruses.
It involves coating these pathogens with specific proteins called “opsonins.”

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5
Q

Which leukocyte is active in both the Nonspecific (innate) and Specific (acquired) Immune responses?
1. B- lymphocyte
2. Macrophage
3. Basophil
4. T-lymphocyte

A
  1. Macrophage
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6
Q

A titer is an example of

A

Direct serology

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7
Q

Blood Typing is an example of
1. Indirect serology
2. Direct serology

A
  1. Direct serology

Why?
We are trying to identify antigens in the patient. Not antigens being made against antibodies.

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8
Q

Looking at the Table below in which throat culture (GABHS) gives the true diagnosis. Is the Centor Score more sensitive or more specific?
1. the test is more sensitive, than specific
2. the test is equally sensitive and specific
3. the test is more specific, than sensitive
4. it can’t be determined from this data

A
  1. the test is more sensitive, than specific

Sensitive: 45 / (45 + 5) = 0.9 = 90%
Specific: 41 / (41 + 9) = 0.82 = 82%

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9
Q

If a veterinary team gives A- (DEA 1.1) donor blood to an A+ recipient dog, what will happen?
1. blood transfusions are not performed in dogs
2. anaphylactic reaction, A+ is the universal donor
3. anaphylactic reaction, the donor and recipient are not of the same blood type
4. should support the patient, A- is a universal donor

A
  1. should support the patient, A- is a universal donor

A+ given to A- will cause anaphylactic reation within 45min

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10
Q

Define “Indirect Serology”
Detect ___ being made by the patient against a specific ___

A
  1. Antibodies
  2. Antigen

“Antibodies against antigen”

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11
Q

Describe how Foal Passive Antibody Transfer test works using antibodies at the molecular level.
(not what steps you performed)

A

Small latex spheres are coated with antigens.
If the sample contains antibodies to that antigen, the spheres will stick together. If they’re not present, the mixture will remain dispersed.

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12
Q

Define “Direct serology”
Uses ___ in the test to identify an ___ in the patient

A
  1. Antibodies
  2. Antigen

“Antibodies to identify antigens”

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13
Q

What do you tell the owners if a test is positive for FeLV? (5)

A
  1. Cat must be isolated (indoor cat now)
  2. Retest in 3 months
  3. Virus may clear from body in 6 weeks
  4. Pt may present as asymptomatic or with systemic illness/neoplasia.
  5. Vaccinate any negative cats in the household
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14
Q

What do you tell the owners if a test is positive for FIV?

A
  1. Isolate (indoor time!)
  2. Pt can be asymptomatic or show signs
    - Fever, weight loss, anemia, recurring infections, &/or gingivitis.
  3. Tx is supportive care &/or antiviral agent
  4. Other cats in household will need to be tested.
  5. If fixed, cats can cont living together
    - Trans thru bites. Fixing lowers bite rates.
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15
Q

How does this technology (ELISA testing) work using antibodies at the molecular level?

A

The enzymes bound to the antibodies imbedded in the test cause a color reaction
(when antibodies are presented?)

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15
Q

Feline blood type A can donate to…

A

Type A or AB

16
Q

What amount of serum & saline would you need for a 1:1 dilution ratio starting with 1mL serum?

A

Serum: 1mL
Saline: 0mL

17
Q

What amount of serum & saline would you need for a 1:2 dilution ratio?
1:10?

A

a. Serum: 1mL Saline: 1mL
b. Serum: 1mL Saline: 9mL

18
Q

What is the formula for a simple dilution?

A

Sample volume / (Sample volume + Diluent volume)

So, 1:2 dilution is 1 part sample & 1 part diluent.
1:10 diluent has 1 part sample & 9 parts diluent.

19
Q

How do dilutions apply to diagnostic titers?

A

Tells us how many antibodies are in the patient

20
Q

Define:
1. Sensitivity
2. Specificity

A
  1. Correctly detect patients who do have said disease.
  2. Correctly detect patients without the said disease.