Cytology/Histology LAB

Question 1

What are important & prominent cytology features that are consistent with inflammation? (5)

Answer 1

1. Pyknosis:
   - Slow cell death
   - Small, condensed, dark nucleus that may be fragmented.
2. Karyolysis:
   - Rapid cell death
   - Occurs with some septic inflammatory reactions
   - Appears swollen, ragged nucleus without intact membrane
3. Bacteria
4. Neutrophils
5. Monocytes

Question 2

What are important & prominent cytology features that are consistent with malignant neoplasia? (8)

Answer 2

1. Anisokaryosis
   - Any unusual variation in the overall size of the cell nucleus.
2. Pleomorphism
   - Variability in size & shape of the same cell type.
3. High nucleus to cytoplasm ratio (H:C)
4. Increased mitotic activity
5. Coarse chromatin
6. Multinucleation
7. Nucleoli vary in size
8. Nuclear molding

Question 3

How to identify benign neoplasia (2)

Answer 3

1. Hyperplasia
   - Increase in number of cells in a tissue
2. No criteria of malignancy present in the nucleus of cells

Question 4

Three possible sites for bone marrow collection in animals

Answer 4

1. Head of femur
2. Head of humerus
3. Ileum of pelvis

Question 5

Incisional biopsy definition

Answer 5

Portion of the tumor is removed

Question 6

Excisional biopsy definition

Answer 6

Entire tumor is removed

Question 7

What type of cell tissue is this?

Answer 7

Epithelial cells

Question 8

What type of cell tissue is this?

Answer 8

Muscle cells

Question 9

What type of cell tissue is this?

Answer 9

Connective cells