Advanced Hematology QUIZ & LAB (LA)

Question 1

In healthy bovine blood, the largest % of leukocytes will be

Answer 1

Lymphocytes

Question 2

A leukocyte that presents antigen to the Specific Immune System (Humoral System)
1. lymphocyte
2. neutrophil
3. monocyte/macrophage
4. eosinophil

Answer 2

3. monocyte/macrophage

Question 3

A special stain to identify reticulocytes is
1. Giemsa stain
2. Wright’s stain
3. New Methylene blue
4. Sudan III

Answer 3

3. New Methylene blue

Question 4

The percentage of whole blood that is composed of erythrocytes is measured by
1. MCH
2. PCV
3. MCHC
4. MCV

Answer 4

2. PCV

Question 5

A Band Cell is
1. a musical cell
2. an immature granulocyte
3. a cancer cell
4. contains lines due to stain artifact

Answer 5

2. an immature granulocyte

Question 6

When evaluating a blood smear, the feathered edge is a good location to evaluate
1. microfilaria
2. large atypical cells
3. all choices are correct
4. platelet clumps

Answer 6

3. all choices are correct

Question 7

Which test differentiates rouleax formation from agglutination?
1. Activated Clotting Time
2. New Methylene Blue Test
3. Cross-match
4. Saline Test

Answer 7

4. Saline Test

Question 8

Band Cells are characterized by
1. size compared to RBCs
2. presence of blue granules
3. Dohle bodies
4. nucleus constriction is < 1/3 of widest part

Answer 8

4. nucleus constriction is < 1/3 of widest part

Question 9

Echinocytes are caused by
1. Lead toxicity
2. Neoplasia
3. EDTA artifact
4. Autoimmune Hemolytic Anemia (AIHA)

Answer 9

3. EDTA artifact

Question 10

Rouleaux formation is normal in which species?
1. can be normal in all of the species listed
2. cow and cat
3. cat and horse
4. dog and horse

Answer 10

3. cat and horse

Question 11

Please identify this equine white blood cell.
1. Reactive lymphocyte
2. Blast cell
3. Toxic band
4. Monocyte

Answer 11

4. Monocyte
   Because:
   Size - 3 RBCs across

Nucleus- horse-shoe shaped

Cytoplasm contains vacuoles - common in this phagocytic cell when it is active

(too large to be toxic band; wrong nucleus to be reactive lymphocyte or blast cell)

Question 12

The presence of toxic neutrophils indicate
1. a stimulus that shortens maturation in the bone marrow.
2. that the animal has been poisoned.
3. that the animal must have a viral infection.
4. that the neutrophils were damaged during smear/staining techniques.

Answer 12

1. a stimulus that shortens maturation in the bone marrow.

Question 13

This image shows…
1. platelet clumping
2. fibrin clumping
3. stain precipitate
4. blood parasites

Answer 13

1. platelet clumping

Question 14

This blood smear shows atypical cells. The vet tech should…
1. skip them because they are not blood cells.
2. record these as cancer cells.
3. record these as large lymphocytes.
4. have slide reviewed by a DVM.

Answer 14

4. have slide reviewed by a DVM.
   Why?
   These very large lymphocytes could be either large reactive OR cancer. A specialist would need to identify (special stains might be needed). Describe what you see in the report, but be certain to ask for a DVM/Pathologist to review!

Question 15

The morphology of this feline RBC is…
1. hypochomasia
2. polychromasia
3. normal
4. immature

Answer 15

1. hypochomasia

Question 16

The arrow is indicating a ___.
1. PMN
2. mast cell
3. toxic band cell
4. monocyte

Answer 16

3. toxic band cell
   Why?
   This immature neutrophil (simple U nucleus) is about the same size as the mature neutrophil in the image (therefore not a monocyte). It shows the open chromatin and large clear granules of and RNA (purple dots) typical of toxic change.

Question 17

Answer 17

“A” shows flame tipped shape/feathered edge and covers at least 2/3 the length of slide.

“D” is also pretty good…shows a little streaking at flame tip.

Question 18

Identify the equine parasite ova

Answer 18

Pinworm ova
(AKA: Oxyuris)

Question 19

Identify the bovine parasite ova

Answer 19

Moniezia expansa

Question 20

Identify the porcine parasite ova

Answer 20

Fasciola eggs