SB3c ii - DNA extraction Flashcards
1
Q
What are the three steps in the DNA Extraction Experiment?
A
- DNA is extracted by mashing/ lysing (breaking) cells. This can be done
using a soap solution that breaks the cell membranes. - Protease enzymes can be used to break down the proteins released when
cells burst. The proteins are not needed. - Cold ethanol is added to cause the DNA to precipitate. DNA is insoluble in ethanol so it cannot remain dissolved in the solution and forms a precipitate.