SB3c ii - DNA extraction

Question 1

What are the three steps in the DNA Extraction Experiment?

Answer 1

1. DNA is extracted by mashing/ lysing (breaking) cells. This can be done
   using a soap solution that breaks the cell membranes.
2. Protease enzymes can be used to break down the proteins released when
   cells burst. The proteins are not needed.
3. Cold ethanol is added to cause the DNA to precipitate. DNA is insoluble in ethanol so it cannot remain dissolved in the solution and forms a precipitate.