S1 [LAB]: Tests for Glucose (Qualitative & Quantitative)) Flashcards
Identify the test for glucose determination:
In hot alkaline solution, glucose reduces copper salts to copper oxide
Benedict’s test
The Benedict’s reagent for glucose testing is made up of what component/s?
Copper sulfate
Sodium citrate
Sodium carbonate
Fehling’s solution A for glucose testing is made up of what component/s?
Cupric sulfate
Fehling’s solution B for glucose testing is made up of what component/s?
Rochelle’s salt
KOH
Give the grading for the Benedict’s and Fehling’s Test for glucose:
No change in color
(-); Negative
Give the grading for the Benedict’s and Fehling’s Test for glucose:
Green opacity and no precipitate
(+/-); Trace
Give the grading for the Benedict’s and Fehling’s Test for glucose:
Green solution with yellow precipitate
(+); Positive
Give the grading for the Benedict’s and Fehling’s Test for glucose:
Green to yellow solution with yellow precipitate
(++); Positive
Give the grading for the Benedict’s and Fehling’s Test for glucose:
Muddy orange solution with yellow precipitate
(+++); Positive
Give the grading for the Benedict’s and Fehling’s Test for glucose:
Orange to brick red precipitate
(++++); Positive
The strong positive reaction of Benedict’s and Fehling’s test for glucose
Orange to brick red ppt.
Identify the method of glucose determination:
In a hot alkaline solution, glucose reduces bismuth salts to metallic bismuth
Nylander’s Test
Positive reaction for the presence of glucose in the Nylander’s test
Black color
Positive reaction for the presence of trace glucose in the Nylander’s test
Brown
In the Nylander’s test for glucose, if the solution turns black after cooling, the reaction is due to ___
substances other than sugar
Identify the glucose determination test:
Caramelization of glucose by strong alkali with the aid of heat
Moore-Heller’s test
Reagent used in the Moore-Heller’s test of glucose determination
10% KOH solution
Give the expected color for the Moore-Heller’s test of glucose determination:
< or equal to 1% sugar
Canary yellow
Give the expected color for the Moore-Heller’s test of glucose determination:
1-2% sugar
Wine yellow
Give the expected color for the Moore-Heller’s test of glucose determination:
2-3% sugar
Cherry color
Give the expected color for the Moore-Heller’s test of glucose determination:
3-4% sugar
Rum color
Give the expected color for the Moore-Heller’s test of glucose determination:
> 4% sugar
Dark brown or black
Identify the test for glucose determination:
When heated with concentrated hydrochloric acid, fructose forms oxymethylfurfurol, which gives a red color with resorcin
Seliwanoff’s Test/ Borchardt’s Test
Seliwanoff’s reagent used for glucose determination is composed of ___
Resorcinol
HCl
Positive result for Seliwannof’s test of glucose determination
Bright red color
Borchardt’s reagent for glucose determination is composed of ___
25% HCl
Resorcinol crystals
Positive result for Borchardt’s test for glucose determination
Yellow ethereal layer
Rubner’s reagent for glucose determination is composed of ___
Lead acetate powder
Concentrated ammonium
Positive result for Rubner’s test of lactose determination
Brick red color
Positive result for Bial Orcinol test of glucose determination
Green color
Identify the sugar being measured:
Benedict’s Test
Glucose
Identify the sugar being measured:
Fehling’ Test
Glucose
Identify the sugar being measured:
Nylander’s Test
Glucose
Identify the sugar being measured:
Moore-Heller’s Test
Glucose
Identify the sugar being measured:
Seliwanoff’s Test
Fructose
Identify the sugar being measured:
Borchardt’s Test
Fructose
Identify the sugar being measured:
Rubner’s Test
Lactose
Identify the sugar being measured:
Bial Orcinol Method
Pentose
Overflow type of glucosuria
Pre-renal
Which reducing sugar is of diagnostic significance?
Galactose
Preferred specimen for fructose determination
First morning urine
Give the ratio of rgts. 1 & 2 in Fehling’s test
1:1 (equal parts)
Components of Nylander’s reagent
Rochelle salt
10% NaOH or KOH
Bismuth subnitrate
