S1 [LAB]: CHEMICAL EXAM OF URINE Flashcards
Testing using reagent strip is performed within ___ after collection
1 hour
Normal pH level of urine
4.5
Optimal temperature for reagent strip storage
below 30C
T/F: Distilled water is used as a negative control in reagent strip
F
pH level of first morning urine
slightly acidic (5-6)
pH level of urine following a meal
alkaline
T/F: An unpreserved urine specimen does not reach pH 9
T
Indicator of pH in a reagent strip
methyl red & bromthymol blue
Reflects the kidneys’ role of compensating against acidosis
Urine pH
Range detected by the pH indicator in the reagent strip
5-9
Samples left standing can have falsely ___ pH as CO2 is lost and bacterial growth produces ammonia from urea
elevated
Reagent strips are only sensitive to this specific protein
Albumin
In respiratory and metabolic acidosis, the urine is expected to be ___
Alkaline
pH level associated with unpreserved specimen
9
The most indicative of renal disease
Protein
Often associated with early renal disease
Protein
The most frequent chemical analysis on urine
Glucose
For the detection & diabetes mellitus monitoring
Glucose
Most valuable in management & monitoring of type-1 DM
Ketones
A deficiency in insulin, indicating the need to regulate dosage (insufficient insulin dosage)
Ketonuria
___ RBCs per microliter of urine is considered clinically significant
> 5
___ provide the most accurate means for determining presence of blood
Hemoglobin testing
Heme-containing protein found in muscle tissue
Myoglobin
Myoglobin produces a clear ___ urine
red-brown
The ____ portion of myoglobin is toxic to the renal tubules; high concentrations can cause acute renal failure.
heme
Primary test for dx, monitoring bacterial infection & evaluate the success of antibiotic
Nitrite
Provides a rapid screening test for the presence of UTI; to detect cases in which urine CS may not be apparent
Nitrite
Requires the longest time of all the reagent strip reaction (2 minutes)
Leukocyte
11th parameter in chemical exam of urine
Ascorbic acid
Principle of ascorbic acid measurement
Tillman’s reaction
Color change in Tillman’s reaction
Blue to green
Component of the Tillman’s reagent
2,6-dichloroindophenol sodium
Components of glucose in reagent strip
Glucose oxidase
Peroxidase
Potassium Iodide
Principle of glucose in reagent strip
Double sequential enzyme reaction
In glucose, the catalyst in the reagent strip is the ___
Glucose oxidase
How many seconds for glucose?
30
T/F: Glucose crosses the placenta, whereas insulin does not.
T
How many seconds for bilirubin?
30
Bilirubin in the reagent strip is based on the Coupling of Bilirubin with diazotized dicloroaniline in a ___ medium
strongly acidic
Component of bilirubin in reagent strip
Dichloroaniline diazonium salt
Color result of bilirubin in reagent strip
Tan»_space; Shade
How many seconds in ketone?
40
Component of ketone in reagent strip
Sodium nitroprusside
Ketone measurement is based on the ___ developed with acetoacetic acid or acetone and nitroprusside
purple color
How many seconds for specific gravity?
45
Component of specific gravity in reagent strip
BTB / polymethylvinyl ether
In specific gravity, BTB produces a ___ color in urine of low ionic concentration
deep blue-green
How many seconds for blood?
60
Component of blood in reagent strip
Tetramethylbenzidine/ Dihydroperoxide
The detection of occult blood is based on the ___-like activity of hemoglobin which catalyze the reaction of peroxide and the tetramethylbenzidine
peroxidase
How many seconds for pH?
60
Color of pH 5.0 in reagent strip
Orange
Color of pH 8.5 in reagent strip
Blue-green
Component of protein in reagent strip
Tetrabromphenol Blue
Negative reaction for protein in reagent strip
Yellow
Positive result of protein in reagent strip
Green»_space; Green
The pigment responsible for the characteristic brown color of feces
Urobilin
How many seconds for urobilinogen?
60
Color of increased urobilinogen level in reagent strip
Red
Component of urobilinogen in reagent strip
p-diethylaminobenzaldehyde
Urobilinogen measurment through the reagent strip is based on ___
Ehrlich reaction
How many seconds for nitrite?
60
Component of nitrite in reagent strip
Tetrahydrobenzoquinolone
Nitrite test using reagent strip is based on ___
Griess reaction
How many seconds for leukocyte?
120
Component of leukocyte in reagent strip
Pyrrole amino acid ester diazonium salt
pH level associated with unpreserved urine
9
Bence jones protein is turbid and clear at what temperatures?
Turbid: 40-60C
Clear: 100C
Amount of RBCs considered clinically significant
> 5 RBCs/ microliter
T/F: If myoglobin is found in the urine, it produces a turbid red-brown color
False: clear red-brown color
Strong positive reaction of bilirubin in reagent strip
Tan
In the Watson- Schwartz test, the chloroform tube and butanol tube will have what respective results in urobilinogen?
Both are miscible
In the Watson- Schwartz test, the chloroform tube and butanol tube will have what respective results in porphobilinogen?
Both are immiscible
Normal value for urobilinogen in the urine
0.2-1.0 Ehrlich units
Normal pH of the urine
5.5-8.0