RPA Cultering Microorganisms

Question 1

Describe process

Answer 1

• agar gel set in a Petri dish
  = bacteria grow to visible colonies

Question 2

How to avoid risks

Answer 2

• sterilise all Petri dishes
  = kills unwanted microorganisms and prevents contamination
• bacteria transferring by using inoculating loop
  = sterilise it before use through a Bunsen burner flame
• once bacteria transferred
  = use a adhesive tape
  = stop lid from falling off and unwanted microorganisms entering
• place agar plate upside down
  = stops moisture from dripping down into bacteria and disrupting colonies

Question 3

Temperature of incubation

Answer 3

Normally incubate at 25C
= reduce changes that harmful bacteria will grow

Question 4

Process of effects of antibiotics on bacterial growth

Answer 4

• clean bench with disinfectant
• sterilise inoculating loop through flame
• open sterile agar gel plate near flame
  = flame kills bacteria in the air
• use loop to spread chosen bacteria evenly over plate
• place sterile filter paper discs containing antibiotic onto plate
• incubate plate at 25C