RNA Transcription

Question 1

RNA poly1

Answer 1

Makes rRNA. Single precursor made into 18s and 28s subunits. Uncapped in nucleolus

Question 2

Rna poly2

Answer 2

Makes hnRNA which is processed to mRNA. Have 5’ end cap

Question 3

RNA poly3

Answer 3

Makes tRNA, which is uncapped

Question 4

DnaG

Answer 4

It’s a primase in prokaryotes

Question 5

Promoters

Answer 5

Required, tata box 25-30 upstream, or ccaat 40-100.

Question 6

Enhancers

Answer 6

Tissue specific positive control. Orientation and distance independent.

Question 7

Repressors

Answer 7

Like enhancers, but negative

Question 8

Pol 2 accessory proteins

Answer 8

TFIID has TBP, all need TBP. Make complex holoenzyme

Question 9

Enhancer binding proteins

Answer 9

Recruit transcription factors, usually bind as dimers. Some need ligands or phosphorylation.

Question 10

Histones

Answer 10

When near promoter, inhibits transcription. When acetylated, transcription increases.

Question 11

What does dna methylation do?

Answer 11

Inhibits transcription. Occurs at cytosine residue