ER And Golgi Flashcards
ER and golgi location
ER form outer nuclear membrane to plasma, golgi near nucleus
Luminal volume of ER golgi is equal to what?
Extra cellular matrix space
ER exit sites
Located in RER, act like SER
ER vs plasma membrane
ER makes phospholipids, even in ER because of scramblase. Plasma has flippase
SER look
No ribosomes, distinct proteins. Leydig cells have a lot.
SER function
Peroxisomes and autophagosomes making, detox drugs, makes steroid hormones, sequester Ca.
RER function
Translation, processing and trafficking of proteins. Innermost RER is outer nuclear membrane
What ribosomes can TEM see?
Free and bound
What do SRP receptors do?
Bind particle to attach ribosome to lumen
Protein types that need ER. All cotranslational
Insertion into lumen
Insertion into ER membrane
Insertion into membrane where either terminus enters
Insertion where both terminus in cytosol
Insertion where multiple domains in and out
Protein charges pulled where?
Toward cytosol
Processing in RER types
Signal peptidase, form disulfide bonds, folding and glycolysation
Vesicular transport steps
Vesicles forms, filled with molecules, addressed and then fuses at new compartment
In depth vesicle
G protein coupled. Makes protein coat and buds off. Forward uses COPII coats, reverse uses COPI coats
COPII coats use what protein to move?
Dynein
