RNA: Nucleic Acids

Question 0

Usually single stranded

Answer 0

Ribonucleic Acid “RNA”

Question 1

Usually double stranded

Answer 1

Deoxyribonucleic Acid “DNA”

Question 2

Stores genetic information

Answer 2

Nucleic Acids

Question 3

DNA is a

Answer 3

Polydeoxyribonucleotide

Question 4

The double stranded DNA with one strand strand sequence of 5’-AGCAGC-3’ has this many total ______.

Answer 4

Purines

Question 5

The double stranded DNA with one strand strand sequence of 5’-AGTAGC-3’ has this many total ______.

Answer 5

Thymines

Question 6

The double stranded DNA with one strand strand sequence of 5’-AGCAGC-3’ has this many total ______.

Answer 6

Hydrogen bonds

Question 7

Twisting of dsDNA

Answer 7

Double helix

Question 8

Double Helix: right-handed. Most famous. With 10 residues per 360 degree turn.

Answer 8

B form

Question 9

Double Helix: left-handed. About 12 residues per turn.

Answer 9

Z form

Question 10

Double Helix: right-handed. Dehydrated B form. 11 residues per turn. Usually found in DNA-RNA or RNA-RNA.

Answer 10

A form

Question 11

Disruption of the Hydrogen bonds. By _______ or ______. Melting temperature. (Tm)

Answer 11

Denaturation. Chemicals or heat.

Question 12

Half of the helical structure is destroyed

Answer 12

Melting Temperature (Tm)

Question 13

Reattachment of complementary strands of DNA to form a double strand

Answer 13

Renaturation/Reannealing

Question 14

The DNA double helix is stabilized by the _________ nature of the stacked nitrogen bases and these reaction.

Answer 14

Hydrophobic

Question 15

DNA Replication is ________. Needs a ______ as template. Needs a lot of ________. Has been studied in E. coli.

Answer 15

Semiconservative. dsDNA. Enzymes.

Question 16

Steps of DNA Replication: 1. Occurs at origin/s of replication. AT-rich region/s. Multiple origins Eukaryotes.

Answer 16

Strand Separation

Question 17

Main enzyme of Strand Separation

Answer 17

DnaA protein

Question 18

Steps of DNA Replication: 2. Occurs at origin/s of replication.

Answer 18

Replication Fork Formation

Question 19

Enzyme for Replication Fork Formation. Binds to single stranded DNA. Moves along the DNA. Forces breakage of hydrogen bond.

Answer 19

DNA Helicase

Question 20

Enzyme for Replication Fork Formation. Binds cooperatively to single-stranded DNA. Prevents reannealing of ssDNA strands. Protects ssDNA from nucleases.

Answer 20

SsDNA-binding proteins (SSB)