RNA Flashcards
Peptide Synthesis
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DNA is stored in _____.
genes
Genes
code for proteins
Proteins
determine what cell will be
____ —> _____ —> _____ —> _____
DNA —-> genes —> proteins —> cell
Stuff about DNA
- stores information in the nucleus (vault) where it never leaves
- instructions copy DNA into RNA
- mRNA directs assembly of proteins
Central Dogma
- belief system
- palm of information
DNA —> mRNA —> protein
Transcription
- language of nucleotides
- mRNA is made from a gene within the DNA
Translation
- amino acids
- mRNA gets used to direct the production of a protein
RNA
- sugars in RNA have extra oxygen
- single strand
- T is replaced by pyrimidine U (uracil)
3 Kinds of RNA`
- messenger (mRNA) snakes through pore
- ribosomal (rRNA) factory
- transfer (tRNA) sugar plum fairy
RNA Polymerase
- produces mRNA copy of DNA during transcription
- moves down DNA and assembles complementary strait of mRNA
- nucleotide sequence signals it to stop
- leaves nucleus and goes to rough ER
Explain Translation
- cells translate info in DNA to language of proteins
- follows rules of genetic cod
- mRNA is read in unites called codons
Codons
- corresponds to a particular amino acid
- 64 different codons
- 20 amino acids
Genetic Code Dictionary
- determined from trial and error experiments to work out which cordons matched with which amino acids
- universal! used by all living things
rRNA Translation
- occurs in ribosomes
- 2 subunits
- small
- large
Small Subunit
- has short sequence of rRNA exposed that is identical to leader sequence
- mRNA binds to small subunit
Large Subunit
- has 3 binding sites the tRNA located directly adjacent to the exposed rRNA sequence on the small subunit
- called A,P,E sites
Complete Ribosome
when mRNA molecule, small subunit, and large subunit all come together
tRNA enter ribosome at _____ site.
A
tRNA
molecules that bring amino acids to ribosome to use in making proteins
tRNA Structure
- amino acid attachment site on one end and 3 nucleotide sequence on the other
- activating enzymes
- anticodon
Activating Enzyme
match amino acids with their proper tRNAs
Anticodon
3 nucleotide sequence complementary to 1 of the 64 codons of genetic code