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bacteriology > Rickettsiaceae > Flashcards

Rickettsiaceae Flashcards

1
Q

Small (0.5 – 2 um) non-motile, pleomorphic bacilli, have a g(-) cell wall

A

RICKETTSIACEAE

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1
Q

It is the simplest
bacterial form and
considered transitional
organism between
bacteria and viruses

A

RICKETTSIACEAE

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2
Q

This group of organisms
infect wild animals, with
humans acting as
accidental hosts

A

RICKETTSIACEAE

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3
Q

all species require living cells for growth except for

A

Bartonella quintana

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4
Q

small organism, pleomorphic, gram-negative bacilli and multiplies by binary fission in the
cytoplasm of host cells (the release of mature rickettsiae results in the lysis of the host cell)

A

Rickettsia

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5
Q

survives only briefly outside of a host and multiply only intracellularly in the cytosol of the host
cell

A

Rickettsia

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6
Q

Rickettsia :
Lysis of cell wall

A

Rickettsia prowazekii

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7
Q

Rickettsia:
Filopodium focal lysis

A

Ricketssia rickettsii

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8
Q

Rickettsia:
Budding

A

Rickettsia tsutsugamushi

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9
Q

contributes to its intracellular activity.

A

Phospholipase A2

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10
Q

can be passed from generation to generation of ticks through their eggs (transovarian
passage)

A

Rickettsia ricketsii

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11
Q

Ricketssia Groups of Bacteria

A

Spotted fever group
Typhus Group
Scrub typhus group

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12
Q

They grow in the cytoplasm of host cell and released via ―pinching‖ off‖ a host cell membranebound rickettsia

A

Orientia tsutsugamushi

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12
Q
  • It was placed into a separate genus due to the absence of LPS and peptidoglycan, and the
    presence of 54 – 58 kDa major surface proteins
A
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13
Q

It is transovarially maintained in mites (Leptotrombidium

A

Orientia tsutsugamushi

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14
Q

Humans and rats are accidental, nonessential dead-end hosts

A

Orientia tsutsugamushi

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15
Q

Small (0.5 um, gram negative coccobacilli and undergo intracellular development cycle following
infection of circulating WBC

A

Ehrlichia

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16
Q

nfection of circulating WBC
- They undergo 3 developmental stages similar to Chlamydiae: EB, initial bodies and morulae

A

Ehrlichia

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17
Q

3 Developmental stages of Erlichia

A

Elementary body, Initial body, Morulae

18
Q

Wright-Giemsa staining of intravacuolar microcolony resembles?

A

“mulberry” morula

19
Q

Infects monocytes and causes HME (Human Monocytic Ehrlichiosis)

A

Erlichia Chaffeensis

20
Q

It is transmitted to humans by the lone star tick (Amblyomma americanum)

A

Erlichia Chaffeensis

21
Q

Can also be transmitted by D. andersoni and Ixodes pacificus

A

Erlichia Chaffeensis

22
Q

Causes HGE (Human Granulocytotropic Ehrlichiosis)

A

Erlichia phagocytophila

23
Q

Transmitted by I. pacificus, I. scapularis, and I. ricinus ticks, and white footed mouse
(Peromyscus leucopus)

A

Erlichia phagocytophila

24
Q

This is the causative agent of Q fever – a systemic infection that affects the lungs

A

Coxiella burnetti

25
Q
  • Strictly intrancellular, gram-negative bacteria, smaller that Rickettsia species; more resistant to
    chemical and physical agents, dessication and sunlight (able to withstand harsh environmental
    conditions)
A

Coxiella burnetti

26
Q

Can survive extracellularly because of its endospore-like body – can be grown only in lung cells

A

Coxiella burnetti

27
Q

In infected animals, organisms are shed in urine, feces, milk and birth products
- Humans are infected by the inhalation of contaminated aerosols from dried animal feces

A

Coxiella burnetti

28
Q

In contrast to rickettsial infection, a rash does not develop following infection

A

Coxiella burnetti

29
Q

In contrast to rickettsial infection, a rash does not develop following infection
- It does not multiply in bacteriologic culture media

A

Coxiella burnetti

30
Q

Shell vial assay with human lung fibroblasts is used to isolate the organism from the buffy coat
and biopsy specimens has not resulted in any laboratory acquired infections

A

Coxiella burnetti

31
Q

serologic techniques for detection of Coxiella burnetti

A

IFA, CF, EIA

32
Q

Animal reservoir – cattle, sheep and goats

A

Coxiella burnetti

33
Q

Facultative intracellular gram-negative bacilli
- Do not synthesize acid from carbohydrates

A

Bartonella

34
Q

They live within red blood cells in their natural mammalian hosts

A

Bartonella

35
Q

They can be cultivated in blood enriched with 5% CO2 (CAP) or charcoal yeast extract agar
(CYEA)

A

Bartonella

36
Q

Trench fever is transmitted from person to louse (Pediculus humanus corporis) to another
person.

A

Bartonella

37
Q

Bartonella species: negative catalase, oxidase and urease

A

Bartonella henselae

38
Q

Bartonella that is an etiologic agent of trench fever

A

Bartonella quintana

39
Q

Bartonella that is an etiologic agent og infective endocarditis

A

Bartonella elizabethae

40
Q

Bartonella that is an etiologic agent of Oraya fever (chronic verruga peruana) and febrile acute hemolytic anemia

A

Bartonella bacilliformis

41
Q

for the detection of morulae during the febrile stage of Ehrlichiosis

A

Giemsa or Diff-Quik stains

42
Q

agglutination of certain strains of Proteus vulgaris by serum from patients
with rickettsial diseases; presumptive test

A

Weil-Felix reaction

43
Q

– excellent sensitivity for detecting antibodies to rickettsia;
early diagnosis of RMSF within 7 – 10 days after onset of symptoms

A

Microimmunofluorescent Dot test

44
Q

– do not induce Weil-Felix antibody in infected patients
- Antibodies to rickettsia (except R. rickettsia) cannot be reliably detected until at least 2 weeks
after the patient has become ill

A
  • Q fever, Ehrlichiosis and Rickettsial pox

bacteriology (24 decks)

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