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bacteriology > Corynebacterium > Flashcards

Corynebacterium Flashcards

1
Q

Also known as the diphtheria bacillus or Kleb-Loffler bacillus

A

C. diph

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2
Q

It is not part of the indigenous microbiota of the respiratory tract,
and only inhabits the human nasopharynx in a carrier state
o carrier state – harbors the bacteria but asymptomatic

A

C.diph

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3
Q

It rarely enters the bloodstream

A

C.diph

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4
Q

It is readily killed by heat and by most usual disinfectants
• It is a glucose and maltose fermenter

A

C.diph

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5
Q

Preferred medium: Regular culture media enriched with
serum, cysteine, and potassium tellurite

A

C.diphth

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6
Q

Its cells have rounded ends and “club-shaped sweiings”
o Its highly pleomorphic cells are arranged in pairs and
create X, V, Y and L formations that closely resembles
Chinese letters

A

C,diph

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7
Q

Biochemical tests: (-) Urease, (+) nitrate reduction

A

C.diph

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8
Q

Best specimens: Nasopharyngeal and throat swabs

A

C.diph

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9
Q

Toxin: It favors an alkaline pH (7.8-8.0), an aerobic
environment, and sufficient amount of iron in the
medium consumed

A

Diph toxin

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10
Q

It causes tissue necrosis and exudate formations over
the tonsils, larynx and pharynx

A

C.diph: diph toxin

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11
Q

t is an acute, infectious disease that is characterized by
the production of a systemic toxin and a false membrane
lining of the throat mucous membrane that may
eventually lead to respiratory obstruction

A

Respiratory diphtheria

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12
Q

o Non-healing ulcers with dirty gray membrane
o Infection of the skin/non-respiratory sites

A

• Cutaneous or skin diphtheria (Veldt sore)

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13
Q

Systemic infection affecting various organs
▪ Demyelinating peripheral neuritis
▪ Cardiac failure Systemic infection affecting various organs
▪ Demyelinating peripheral neuritis
▪ Cardiac failure

A

Cutaneous or skin diphtheria (Veldt sore)

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14
Q

• It is one of the most frequently isolated and most clinically
significant Corynebacteria species

A

C. urea

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15
Q

It is a urinary pathogen, a strict aerobe, and lipophilic

A

C.urea

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16
Q

It does not ferment glucose and maltose

A

C.urea

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17
Q

Microscopy: Arranged in V-shaped forms and palisades

A

C.urea

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18
Q

Culture: BAP - Colonies are pinpoint, white, smooth, and are
non-hemolytic

A

C.urea

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19
Q

Biochemical test: Rapid urease producer

A

C.urea

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20
Q

It is an indigenous microbiota of the human nasopharynx
o indigenous microbiota
▪ normal inhabitants but limited population
▪ not easy to isolate

A

C.pseudo

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21
Q

It causes respiratory infection, UTI, and cutaneous wound
infections in immunocompromised patient, such as those who
have AIDS

A

C.pseudo

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22
Q

Microscopy: Cells are arranged in parallel rows or palisades
and do not exhibit any other characteristic “pleomorphism” that
is similar to other corynebacteria

A

C.pseudo

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23
Q

Biochemical test: (+) Urease and nitrate production

A

C.pseudo

24
Q

It is a skin microbiota that is found in inguinal, axillary, and rectal
sites

A

Corynebacterium jeikeium

25
Q

It is an obligate aerobe and a multi-antibiotic resistant bacterium

A

C.jeikye

26
Q

It is isolated from immunocompromised individuals

A

C.jeik

27
Q

It is a common cause of diphtheroid prosthetic valve
endocarditis in adults

A

C.jeik

28
Q

Microscopy: Pleomorphic, club-shaped, and arranged in V-
shaped forms

A

C.jeik

29
Q

BAP - Colonies appear large when added with 1%
Tween 80

A

C.jeik

30
Q

Biochemical test: (-) Urease and nitrate reduction.

A

C.jeik

31
Q

It is acquired through animal contact or ingestion of
unpasteurized dairy products

A

C.ulcer

32
Q

It is also isolated from skin ulcers and exudative pharyngitis

A

C.ulcer

33
Q

• It is associated with diphtheria-like sore throats since a
significant isolates have shown to produce a diphtheria-like
toxin

A

C.ulcer

34
Q

BAP - Colonies have a narrow zone of Beta hemolysis
o CTBA (Cysteine-Tellurite Blood Agar) - Colonies have a
surrounding brown halo
o Loeffler’s serum agar - Colonies exhibit growth

A

C.ulcer

35
Q

Biochemical test: (+) Urease and gelatinases, (-) nitrate
reduction

A

C.ulcer

36
Q

It produces a dermonecrotic toxin that causes death of
various cell types

A

C.pseudotuberculosis

37
Q

CTBA - Colonies exhibit a black color and are
surrounded with a brown halo
o BAP - Colonies are small and yellowish-white

A

C.pseudotuberculosis

38
Q

Biochemical test: (+) Urease (-) gelatinase

A

C.pseudotuberculosis

39
Q

Methylene blue staining: Bacterial cells exhibit a
beaded formation

A

C.diph

40
Q

are used for staining
metachromatic granule

A

Neisser’s and Albert’s stains

41
Q

staining method is composed mainly
of either methylene blue or crystal violet

A

Neisser’s staining

42
Q

stain is composed of malachite green
and toluidine

A

Albert’s stain

43
Q

It is the preferred medium for the isolation and
identification of corynebacteria

A

Cytine tellutite blood agar (CTBA)

44
Q

The multiplication of corynebacteria occurs
within the range of what degree celsius?

A

15°C to 40°C

45
Q

+) Result: Colonies of corynebacteria exhibit a
black or brown color after 48 hrs of incubation

A

CTB

46
Q

It contains sheep blood, bovine serum, cysteine,
and potassium tellurite

A

CTBA

47
Q

It is composed of sheep blood, cysteine,
potassium tellurite, and sodium thiosulfate

A

Tindsdale agar

48
Q

gram-positive and urease negative with brown halo

A

C. diphtheriae

49
Q

It is useful for observing the microscopic
morphology and metachromatic granules of C.
diphtheriae

A

Loeffler’s serum agar

50
Q

▪ (+) Result: C. diphtheriae colonies exhibit a
“poached-egg” appearance

A

o Loeffler’s serum agar

51
Q

The metachromatic granules of C. diphtheriae
are called

A

“Babes-Ernst” bodies

52
Q

This is used to observe the urease production of
C. uralyticum

A

Christensen urea slant

53
Q

In vitro diphtheria toxin detection using
immunodiffusion

A

Elek’s Test

54
Q

Precipitin lines are observed
- slant lines found in the junction where the
growth of inoculum and filter paper meet

A

Elek’s Test

55
Q

All staph are Aerobic or facultative anaerobic except for

A

S. saccharolyticus

bacteriology (24 decks)

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