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bacteriology > Gram - negative bacilli, non spore forming part 2 > Flashcards

Gram - negative bacilli, non spore forming part 2 Flashcards

1
Q

It is also known as the ―”plague bacillus”

A

Yersinia pestis

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2
Q

It is a class A bioterrorism agent

A

Yersinia pestis

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3
Q

It is the only enterobacterium that is transmitted to humans through the bite of an infected flea

A

yersinia pestis

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4
Q

It is the causative agent of the bubonic plague

A

yersinia pestis

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5
Q

It can be isolated on a routine culture media, and grows best at 25C to 30C

A

Yersinia pestis

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6
Q

Vector of Yersinia pestis

A

Xenopsylla cheopis (oriental rat flea)

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7
Q

Its reservoir is the rats

A

Yersinia pestis

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8
Q

Virulence factors of Yersinia pestis

A

Endotoxin, coagulase, and fibrinolysin

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9
Q

Microscopy: Short, plump rod with a bipolar body or a ―”closed safety pin” appearance

A

Yersinia pestis

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10
Q

Broth- Colonies have a ―stalactite-shaped‖ pattern

A

Yersinia pestis

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11
Q

It is a disease of the rodents that is caused by Yersinia pestis and is transmitted to humans
through flea bites

A

Plague

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12
Q

Humans may also acquire it by ingestion of contaminated animal tissues and inhalation of
contaminated airborne droplets

A

Plague

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13
Q

Once plague is inside the human body, the bacteria multiply in the?

A

blood and lymph

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14
Q

There are 2 forms of plague:
It results from the bite of infected flea

A

Bubonic plague

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15
Q

There are 2 forms of plague:
It is associated with high fever and painful inflammatory swelling of the axilla and
groin

A

Bubonic plague

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16
Q

There are 2 forms of plague:
It is acquired by close contact with infected individuals

A

Pulmonary plague

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17
Q

There are 2 forms of plague:
It occurs secondarily to bubonic plague

A

Pulmonary plague

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18
Q

It is the most commonly isolated species of Yersinia

A

Yersinia enterocolitica

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19
Q

It is the causative agent of enterocolitis or waterborne gastroenteritis

A

Yersinia enterocolitica

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20
Q

It is motile in SIM at 22C but not at 35C

A

Yersinia enterocolitica

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21
Q

It has been isolated from contaminated packed RBC units, and is thus considered as a blood
transfusion hazard

A

Yersinia enterocolitica

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22
Q

It has the ability to survive in cold temperatures

A

Yersinia enterocolitica

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23
Q

Its reservoirs are swines, dogs, cats, rabbits, and cows

A

Yersinia enterocolitica

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24
Q

Related infections: Appendicitis-like syndrome, arthritis, and erythema nodosum

A

Yersinia enterocolitica

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25
Q

Selective medium: Cefsulodin-irgasan-novobiocin (CIN) aga

A

Yersinia enterocolitica

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26
Q

Microscopy: Coccobacilli with bipolar bodies

A

Yersinia enterocolitica

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27
Q

CIN- Colonies exhibit a ―”bull‘s eye” appearance or dark red or burgundy centers with
transparent borders at 48 hours of incubation

A

Yersinia enterocolitica

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28
Q

It is a pathogen of the rodents, particularly guinea pigs

A

Yersinia pseudotuberculosis

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29
Q

It is motile in SIM at 18C to 25C but not at 35C

A

Yersinia pseudotuberculosis

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30
Q

Its reservoirs are farm and domestic animals (usually birds)

A

Yersinia pseudotuberculosis

31
Q

It is the only oxidase-positive member of the Enterobacteriaceae

A

Plesiomonas shigelloides

32
Q

Its motility is attributed to its polar flagella

A

Plesiomonas shigelloides

33
Q

It has been associated with HIV-positive individuals with inflammatory bowel disease

A

Plesiomonas shigelloides

34
Q

It has been associated with HIV-positive individuals with inflammatory bowel disease

A

Plesiomonas shigelloides

35
Q

Mode of acquisition: Ingestion of undercooked seafood and contaminated water

A

Plesiomonas shigelloides

36
Q

Vibriostatic test: O/129: Sensitive

A

Plesiomonas shigelloides

37
Q

Microscopy: Straight bacilli which can occur singly, in pairs, in short chains, or filamentous

A

Plesiomonas shigelloides

38
Q

o Inositol-brilliant green-bile salt agar: Colonies exhibit white or green to pink color for
other enterics

A

Plesiomonas shigelloides

39
Q

The use of?? enhances the recovery of plesiomonads from
specimen

A

inositol-brilliant green bile agar

40
Q

Decarboxylase test: Positive trio decarboxylase test

A

Plesiomonas shigelloides

41
Q

The species of this genus have been isolated from cold-blooded and warm-blooded animals

A

Edwardsiella

42
Q

Species of Edwarsiella

A

Edwardsiella tarda

43
Q

stain that can be used to obseve the bipolar bodies of Y. pestis

A

Wayson stain

44
Q

Optimal temperature of growth of Serratia and Yersinia?

A

1 to 5 degrees celsius

45
Q

Optimal temperature of growth of Escherichia coli

A

40 degrees celsius

46
Q

Rapid lactose fermenters:

A

Enterobacter, Eschericihia coli, Klebsiella

47
Q

Late lactose fermenters:

A

Citrobacter freundii, Shigella sonnei, and Serratia

48
Q

Non-lactose fermenters:

A

Citrobacter koseri, Edwardsiella, Morganella, Proteus,
Providencia, Slmonella, Shigella, and Yersinia

49
Q

It contains bile salt, bromthymol-blue dye, alicin, lactose, and sucrose
The bile and dye inhibit the growth of other Gram-negative bacilli in the GIT and
promote the isolation of Salmonella and Shigella

A

Hektoen enteric agar (HEA)

50
Q

pH indicator of hektoen enteric Agar

A

Bromthymol blue

51
Q

H2S indicator of Hektoen Enteric Agar

A

Ferric ammonium citrate

52
Q

Growth on HEA
Lactose Fermenter?

A

colonies exhibit a yellow color

53
Q

Growth on HEA
Non-lactose fermenter

A

colonies exhibit a blue-green color

54
Q

Growth on HEA
Non-enteric pathogens

A

-Colonies exhibit an orange to pinkish orange color

55
Q

It is used for the isolation of Salmonella and Shigella species from heavily
contaminated specimens such as stool

A

Xylose-lysine deoxycholate (XLD) agar

56
Q

pH indicator of XLD agar

A

Phenol red

57
Q

H2S indicator of XLD agar

A

sodium thiosulfate and ferric ammonium sulfate

58
Q

Growth on XLD agar shigella?

A

colonies exhibit a pink to red color

59
Q

Growth on XLD agar salmonella?

A

colonies exhibit a pink to red color with black centers

60
Q

Growth on XLD agar other enterobacteriaciae?

A

Colonies exhibit a yellow to red color

61
Q

pH indicator of EMB?

A

eosin and methylene blue

62
Q

result of EMB
Lactose fermenter?

A

Colonies of Escherichia coli exhibit a greenish metallic sheen while those of other exhibit purple color

63
Q

Result of EMB Other coliforms

A

colonies exhibit a pink color

64
Q

Salmonella-shigella agar contents that inhibits gram positive bacteria and some lactose fermenters that are found in stool specimens

A

Bile salts and Brilliant green dye

65
Q

pH indicator of SSA

A

Neutral red

66
Q

H2S indicator of SSA

A

Sodium thiosulfate and ferric ammonium citrate

67
Q

Salmonella serotypin

A

Heat Labile antigen
Flagellar antigen
H antigen

68
Q

Salmonella serotype typhi also produce what

A

Heat labile capsular polysaccharide V-antigen and carries the D serogroup

69
Q

E. coli serotype that are most frequently associated with infantile diarrhea

A

E.coli O55, O111, O127

70
Q

Salmonella-Shigella agar (SSA) - Colonies are pink or colorless with black centers

A

Salmonella

71
Q

Salmonella-Shigella agar (SSA)-Colonies are pink or colorless without black centers

A

shigella

72
Q

Salmonella-Shigella agar (SSA);H2S indicator

A

Sodium thiosulfate and ferric ammonium citrate

73
Q
A

bacteriology (24 decks)

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