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bacteriology > Chkamydiaeceae > Flashcards

Chkamydiaeceae Flashcards

1
Q

They contain both the DNA and RNA, ribosomes and they produce their own proteins, lipids and
nucleic acids
o They were once thought to be large viruses, but they contain both DNA and RNA

A

CHLAMYDIACEAE

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2
Q

Chlamydiaceae stain poorly with gram stain, but they could be better visualized with the

A

Gutsein Stain

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3
Q

Non-motile, small (0.2 – 1.5um), resembles gram-negative cell wall, obligate intracellular
bacteria that requires living cells for growth

A

CHLAMYDIACEAE

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4
Q

Requires the biochemical resources of the eukaryotic host cell‘s environment to fuel their
metabolism for growth and replication – “energy parasite”

A

CHLAMYDIACEAE

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5
Q

They have tropism for columnar epithelial cells

A

CHLAMYDIACEAE

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6
Q

Posses a heat stable, family specific antigen that is an essential component of the cell
membrane (LPS

A

CHLAMYDIACEAE

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7
Q

Morphologic forms of Chlamydiaceae:
Replicative form
Intracellular and metabolically active

A

Reticulate body

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8
Q

Morphologic forms of chlamydiaceae:
Inefective form
Extracellular and structurally resembles a gram-negatice bacillus
Have a rigid cell wall

A

Elementary body

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9
Q

2 components of the outer membrane of elementary body

A

MOMP( major aouter membrane protein and lipopolysaccharide (LPS) antigen

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10
Q
  • Major cause of pelvic inflammatory disease(PID) and ocular trachoma
A

Chlamydia trachomatis

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11
Q
  • Major cause of pelvic inflammatory disease(PID) and ocular trachoma
A

Chlamydia trachomatis

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12
Q

Serotype of endemic trachoma

A

Serotypes A, B, Ba, C

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13
Q

serotype of lymphogranuloma venereum (LGV)

A

Serotypes L1, L2, L3

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14
Q

serotype of PID, urethritis, cervicitis, epididymitis, inclusion conjunctivitis

A

Serotypes D- K

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15
Q

Associated with infertility and ectopic pregnancy

A

Chlamydia trachomatis

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16
Q

Intraperitoneal spread may cause peritonitis or perihepatitis

A

(Fitz-Hugh-Curtis syndrome)

17
Q

Chronic inflammation of the conjunctiva leading to blindness
o It can cause distortion of the eyelids – the eyelashes become misdirected and turn in
o It is transmitted by contact with individuals that carries the organism from an infected
eye, or by flies

A

Trachoma

18
Q

A sexually transmitted disease and has multisystem involvement
Page 223 of 227
o A small painless ulcer or papule appears initially and nodules (buboes) develop after
several weeks
o Fret‘s test – intradermal skin test

A

Lymphogranuloma Venereum (LGV

19
Q

It is characterized by copious discharge from the eye, an inflamed and swollen
conjunctiva, and the presence of large inclusion bodies in the host cell cytoplasm
o Usually affects infants

A

Inclusion conjunctivitis

20
Q

Genus specific antigen can be detected by this methd
 Used to diagnose LGV but not trachoma, inclusion conjunctivitis and
neonatal infection
 Single point titer > 1:64

A

 Complement fixation

21
Q

Used for type-specific antibodies of C. trachomatis
 Can be used for diagnosis of LGV, trachoma, inclusion conjunctivitis and
neonatal infection
 IgM titer of 1:32

A

Microimmunofluorescence assay (Micro-IF)

22
Q

RAtio of single point titer

A

> 1:64

23
Q

Ratio of IgM titer

A

1:32

24
Q

Etiologic agent of psittacosis/ornithosis

A

Chlamydophila (formerly Chlamydia) psittaci

25
Q

Endemic pathogen of all bird species – parrots, parakeets, chicken, ducks

A

Chlamydophila (formerly Chlamydia) psittaci

26
Q

Outbreaks have occurred among turkey-processing workers and pigeon aficionados
- Humans acquire the disease by inhalation of infected aerosols form dried bird excreta or
handling of infected birds
- only laboratories with biosafety level 3 facilities can perform cultivation of samples

A

Chlamydophila (formerly Chlamydia) psittaci

27
Q

widely used serologic test to detect psittacosis

A

. complement fixation

28
Q

sensitive method

A

Direct Microimmunofluorescence

29
Q

identity and distinguish all 9
chlamydial species including C. psittaci

A

Amplification of rDNA using PCR followed by RFLP

30
Q

human pathogen
- transmission is by aerosol droplets via the respiratory route
- “pear -shaped” appearance of EB; has round and dense inclusions

A

Chlamydophila (formerly chlamydia) pneumoniae (TWAR strain)

31
Q

resistant to sulfonamides
- negative for glycogen staining of inclusion bodies
- uses HL of Hep-2 cell lines for culture

A

Chlamydophila (formerly chlamydia) pneumoniae (TWAR strain)

bacteriology (24 decks)

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