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bacteriology > Gram Positive Bacilli, non- encapsulated, non-motile, non-spore forming > Flashcards

Gram Positive Bacilli, non- encapsulated, non-motile, non-spore forming Flashcards

1
Q

Majority of the species are found as indigenous microbiota on the skin and mucous membranes
of humans and animals

A

Corynebacterium

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2
Q

corynebacterium species are glucose and maltose fermenters except

A

C. urealyticum and C.
pseudodiphtheriticum

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3
Q

Slightly curved, gram-positive rods with unparallel sides and slightly wider ends that
produce a ―club-like shape

A

Corynebacterium

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4
Q

BAP-colonies have a small zone of Beta hemolysis, although some strains nonhemolytic

A

Corynebacterium

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5
Q

The members of this group are fastidious corynebacteria that require 48 hours of
incubation before any growth can be observed
 The addition of lipids in the culture medium can enhance the bacterial growth

A

. Lypophilic corynebacteria

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6
Q

Examples of lipophilic corynebacteria

A

Corynebacterium jeikeium and C. urealyticum

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7
Q

The members of this group exhibit fermentative or oxidative metabolism

A

. Non-lipophilic corynebacteria

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8
Q

examples of non-lipophilic corynebacteria

A

: C. diphtheria, C.pseudodiphtheriticum, C. pseudotuberculosis,C.
ulcerans

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9
Q

o Corynebacterium species that are associated with human infections and diseases.

A

: C. diphtheria, C. jeikenum, C. pseudotuberculosis,
C.pseudodiphtheriticum, and C. urealyticum

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10
Q

It is also known as the diphtheria bacillus or Kleb-Loffler bacillus

A

Corynebacterium diphtheria

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11
Q

It is a non-lypophilic, facultative anaerobe but grows best under aerobic conditions
 It is not part of the indigenous microbiota of the respiratory tract, and only inhabits the human
nasopharynx in a carrier state

A

Corynebacterium diphtheria

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12
Q

 It is acquired through inhalations of contaminated respiratory droplets or direct contact with
infected cutaneous lesion

A

Corynebacterium diphtheria

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13
Q

it rarely enters the bloodstream

A

Corynebacterium diphtheria

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14
Q

 It is a glucose and maltose fermenter

A

Corynebacterium diphtheria

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15
Q

Its cells have rounded ends and ―club-shaped sweiings‖
o Its highly pleomorphic cells are arranged in pairs and create X, V, Y and L formations
that closely resembles Chinese letters

A

Corynebacterium diphtheria

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16
Q

Corynebacterium diphtheria biochemical test

A

(-) Urease (+)nitrate reduction

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17
Q

Three biotypes of Corynebacterium diphteriae:
very small, flat, dry, and grayish-black colonies; non-hemolytic

A

Intermedius

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18
Q

Three biotypes of Corynebacterium diphteriae:
small, black, and covex colonies that have a ―”fried-egg” appearance; hemolytic

A

mitis

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19
Q

Three biotypes of Corynebacterium diphteriae:
large, flat, and dark gray colonies that have a ―”daisy-head” appearance; nonhemolytic

A

Gravis

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20
Q

o It is a heat-labile polypeptide
o It is produced by strains with a lysogenic beta-phage that carries the TOX gene

A

Diphtheria Toxin

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21
Q

It causes tissue necrosis and exudate formations over the tonsils, larynx and pharynx

A

Diphtheria Toxin

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22
Q

It favors an alkaline pH (7.8-8.0), an aerobic environment, and sufficient amount of iron
in the medium consumed

A

Diphtheria Toxin

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23
Q

the toxin is absorbed systemically but is less severe

A

. Cutaneous or skin diphtheria (Veldt sore)- C. diptheria

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24
Q

It is one of the most frequently isolated and most clinically significant Corynebacteria species

A

Corynebacterium urealyticum

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25
Q

It is a urinary pathogen, a strict aerobe, and lipophilic

A

Corynebacterium urealyticum

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26
Q

It does not ferment glucose and maltose

A

Corynebacterium urealyticum

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27
Q

Arranged in V-shaped forms and palisades

A

Corynebacterium urealyticum

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28
Q

: BAP- Colonies are pinpoint, white, smooth, and are non-hemolytic

A

Corynebacterium urealyticum

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29
Q

Biochemical test: Rapid urease producer

A

Corynebacterium urealyticum

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30
Q

(Hoffman bacillus)

A

Corynebacterium pseudodiptheriticum

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31
Q

It is an indigenous microbiota of the human nasopharynx

A

Corynebacterium pseudodiptheriticum

32
Q

 It causes respiratory infection, UTI, and cutaneous wound infections in immunocompromised
patient, such as those who have AIDS

A

Corynebacterium pseudodiptheriticum

33
Q

Cells are arranged in parallel rows or palisades and do not exhibit any other
characteristic ―pleomorphism‖ that is similar to other corynebacteria

A

Corynebacterium pseudodiptheriticum (Hoffman bacillus)

34
Q

(+) Urease and nitrate production

A

Corynebacterium pseudodiptheriticum (Hoffman bacillus)

35
Q

It is a skin microbiota that is found in inguinal, axillary, and rectal sites

A

Corynebacterium jeikenum

36
Q

It is an obligate aerobe and a multi-antibiotic resistant bacterium

A

Corynebacterium jeikenum

37
Q

It is isolated from immunocompromised individuals

A

Corynebacterium jeikenum

38
Q

It is a coomon cause of diphtheroid prosthetic valve endocarditis in adults

A

Corynebacterium jeikenum

39
Q

Microscopy: Pleopmorphic, club-shaped, and arranged in V-shaped forms

A

Corynebacterium jeikenum

40
Q

BAP- Colonies appear large when added with 1% Tween 80

A

Corynebacterium jeikeium

41
Q

(-) Urease and nitrate reduction.

A

Corynebacterium jeikenum

42
Q

It is acquired through animal contact or ingestion of unpasteurized dairy products
 It is also isolated from skin ulcers and exudative pharyngitis

A

Corynebacterium ulcerans

43
Q

It is associated with diphtheria-like sore throats

A

Corynebacterium ulcerans

44
Q

CTBA- Colonies have a surrounding brown halo

A

Corynebacterium ulcerans

45
Q

BAP- Colonies have a narrow zone of Beta hemolysi

A

Corynebacterium ulcerans

46
Q

Loeffler‘s serum agar- Colonies exhibit growth

A

Corynebacterium ulcerans

47
Q

(+) Urease and gelatinases, (-) nitrate reduction

A

Corynebacterium ulcerans

48
Q

It is an animal pathogen that humans can contract through direct contact with infected animals

A

Corynebacterium pseudotuberculosis

49
Q

It produces a dermonecrotic toxin that causes death of various cell types
 It can also produce diphtheria toxin

A

Corynebacterium pseudotuberculosis

50
Q

CTBA- Colonies exhibit a black color and are surrounded with a brown halo

A

Corynebacterium pseudotuberculosis

51
Q

BAP-Colonies are small and yellowish-white

A

Corynebacterium pseudotuberculosis

52
Q

(+) Urease (-) gelatinase

A

Corynebacterium pseudotuberculosis

53
Q

best specimens for isolation of C. diptheriae

A

Nasopharyngeal and throat swabs

54
Q

Swab preferred for collection

A

calcium alginate swab

55
Q

: Bacterial cells exhibit a beaded formation

A

Methylene blue statining

56
Q

used for staining metachromatic granule

A

Neisser‘s and Albert‘s stains

57
Q

Neisser‘s staining method is composed mainly of either

A

r methylene blue or crystal violet

58
Q

the components of Albert‘s stain.

A

Malachite green and toluidine

59
Q

CTBA and Tinsdale agar should be incubated for at least

A

48 hours at 35C

60
Q

The multiplication of corynebacteria occurs within the range of

A

15C to 40C

61
Q

 It is the preferred medium for the isolation and identification of
corynebacteria

A

Cytine tellutite blood agar (CTBA)

62
Q

It is a modification of Tinsdale agar
 It is both a selective and differential medium

A

Cytine tellutite blood agar (CTBA)

63
Q

It contains sheep blood, bovine serum, cysteine, and potassium tellurite

A

Cytine tellutite blood agar (CTBA)

64
Q

Colonies of corynebacteria exhibit a black or brown color after
48 hrs of incubation

A

Cytine tellutite blood agar (CTBA)

65
Q

(+) Black or brown colonies surrounded by a brown halo

A

CTBA: C. diphtheria, C.
ulcerans, and C. pseudotuberculosis

66
Q

(+) Result: Colonies exhibit a black colorand are surrounded by abrown
halo

A

Tinsdale agar

67
Q

It is useful for observing the microscopic morphology and metachromatic
granules of C. diphteriae

A

Loeffler‘s serum agar

68
Q

(+) Result: C. diphteriae colonies exhibit a ―”poached-egg” appearance

A

Loeffler‘s serum agar

69
Q

The metachromatic granules of C. diptheriae are called

A

―Babes-Ernst
bodies

70
Q

This is used to observe the urease production of C. uralyticum

A

Christensen urea slant

71
Q

it is a skin test used to determine the susceptibility of a person to diphtheria

A

. Schick test

72
Q

The procedure involves the intradermal introduction of a small amount of diphtheria toxin
into the arm of the individual who is suspected to be harboring the disease
o (+) Result: Redness and Swelling around the site

A

Schick test

73
Q

 It produces a dermonecrotic toxin that causes death of various cell types

A

Corynebacterium pseudotuberculosis

74
Q
A
74
Q
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74
Q
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bacteriology (24 decks)

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