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bacteriology > Agars > Flashcards

Agars Flashcards

1
Q

Differentiation using hemolytic properties

A

BAP

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2
Q

BAP component?

A

Tryptic Soy Agar + 5% Sheep Blood/ 5% Horse Blood

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3
Q
  • used to better isolate Streptococcus pneumoniae.
A

Gentamicin Blood Agar (GBA)

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4
Q

GBA component?

A

TSA+ 5% sheep blood +gentamicin

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4
Q

Differential of MAC

A

Fermentation of lactose

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4
Q

antibiotic inhibitor used to better isolate
Haemophilus spp.

A

Bacitracin-

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5
Q

Makes use of horse blood (High concentration of X and V
Factor)

A

Bacitracin Blood Agar (BCA)

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6
Q

MAC contains what to inhibit growth of gram pos bacteria?

A

Bile salts and crystal violet

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6
Q

V factor

A

NAD or NADP

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6
Q

CAP supplement?

A

VItalex

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6
Q

X factor?

A

Hemin

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6
Q

H2S indicator of SSA?

A

Ferric citrate

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7
Q

indicator of SSA?

A

Neutral red

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7
Q

Indicator of Mac Conkey agar?

A

neutral red

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8
Q

A modified deoxycholate agar

A

Salmonella shigella agar

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9
Q

Inhibitor of SSA

A

Bile salts, sodium citrate and brillant green

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9
Q

-Broth for the Isolation of Shigella and Salmonella

A

. SELENITE F broth

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9
Q

Medium for urine specimens?

A

BAP and MAC

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10
Q

-Strictly anaerobic bacteria (ie, those that cannot grow in the
presence of oxygen) grow at the bottom of the broth.

A

FLUID THIOGLYCOLLATE (FT)

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10
Q

Inhibitors of Thiosulfate citrate Bile Salt sucrose Agar?

A

Bile salt, Sodium cholate, alkaline pH

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10
Q

Agar selective for vibrio spp

A

TCBS agar

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10
Q
  • Used for antibiotic susceptibility testing (Disk Diffusion,
    test, Modified Hodge test, etc.) of most non-fastidious
    bacteria.
A

Mueller Hinton Agat

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10
Q

Standard depth of MHA?

A

4mm

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10
Q

pH pf MHA?

A

7.2-7.4

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11
Q

Indicator of TCBS agar?

A

Bromthymol blue

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11
Q

-General purpose medium that is routinely used to grow
bacteria which tend to have high nutritional requirements
(ce, they are fastidious)

A

TRYPTIC SOY BROTH (TSB)

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11
Q

broth medium for the enrichment of Vibrio species from
food, water, and clinical samples.

A

ALKALINE PEPTONE WATER

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12
Q
  • Used for antibiotic susceptibility testing of Haemophilus
    species.
A

HAEMOPHILUS TEST MEDIUM (HTM)

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12
Q

Medium for stool

A

MAC, SSA,TCBS, Selenite F, APW,

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12
Q

-This broth supports the growth of anaerobes, aerobes,
microaerophilic, and fastidious microorganisms.

A

FLUID THIOGLYCOLLATE (FT)

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13
Q

Strict aerobic bacteria (ie, require oxygen for growth), such
as Pseudomonas sp., tend to grow toward the surface of the
broth

A

FLUID THIOGLYCOLLATE (FT)

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14
Q

-inhibitory for coliforms and certain other microbial specs,
such as focal streptococci, present in fecal specimens

A

SELENITE F Broth

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14
Q
  • Culture broth to grow aerobic and facultative anaerobic
    bacteria.
A

TRYPTIC SOY BROTH (TSB)

14
Q

alkaline pH of this medium allows the growth of Vibrio
organisms while inhibiting the growth of commensal
intestinal bacteria.

A

ALKALINE PEPTONE WATER

15
Q

medium for exudates?

A

BAP, MAC, FT

15
Q

SIM: + for sulfide?

A

Black coloration

15
Q

Medium for transudates?

A

BAP, CAP, MAC, TSB

15
Q

Mediums for Respiratory specimens?

A

BAP, GBA,BCA,MAC

15
Q

Triple Sugar Iron Agar (TSIA) compostion

A

0.1% glucose, 1% sucrose, 1% lactose

15
Q

SIM; + for indole?

A

Reddish pink ring at junction

15
Q

Indicator of TSIA?

A

Phenol Red

15
Q

A/A?

A

Serratia
Escherichia
Enterobacter
Klebsiella

16
Q

SIM rgt?

A

Kovac’s reagent (para-dimethylaminobenzaldehyde)

16
Q

TSIA H2S indicator?

A

Ferrous sulfate

16
Q

K/A:

A

Shigella
Yersinia
Citrobacter
Providencia
Proteus
Yersinia

16
Q

A/A H2S+?

A

Citrobacter
Arizona
Proteus

16
Q

K/A H2S+

A

Citrobacter
Arizona
Salmonella
Edwardsiella

16
Q

Tests H2S production, indole reaction, and motility.

A

SIM (SULFIDE INDOLE MOTILITY)

16
Q

K/K

A

Pseudomonas
Acinetobacter

17
Q
A
18
Q

SIM: + for motility?

A

spread out/ movement away from the stab line/
hazy appearance

19
Q

used to determine the ability of an organism to utilize
sodium citrate as its only carbon source and inorganic
ammonium salts as its only nitrogen source

A

CITRATE

20
Q

Citrate slant: Carbon source?

A

Sodium citrate

21
Q

Citrate slant: nitrogen source?

A

inorganic Ammonium salts

22
Q

pH indicator of citrate?

A

Bromthymol blue

23
Q

+ result for citrate?

A

green to blue slant

24
Q
  • used to determine whether a gram negative rod
    decarboxylates or deaminates lysine and forms H2S
A

Lysine Iron Agar

25
Q
A
25
Q

pH indicator of LIA?

A

Bromcresol Purple

26
Q

Components of LIA?

A

Lysine, peptones, glucose, ferric ammonium
citrate and sodium thiosulfate

27
Q

Positive result for oxidase test?

A

Purple

27
Q

LIA decarboxylation?

A

Butt

27
Q

LIA Deamination?

A

Slant

27
Q

-Tests a microorganisms ability of hydrolyzing urea to
produce ammonia and carbon dioxide

A

Urease test

27
Q

API (ANALYTICAL PROFILE INDEX) principle?

A

Turbidimetry

28
Q

Urease test + color?

A

Pink color change

28
Q

-To differentiate Enterobacteriaceae from
non- Enterobacteriaceae

A

Oxidase test

28
Q

-determine the sensitivity or resistance of pathogenic aerobic
and facultative anaerobic bacteria to various antimicrobial
compounds.

A

KIRBY-BAUER DISK DIFFUSION

28
Q

Oxidase test detects the presence of?

A

Cytochrome oxidase

28
Q
  • Gram positive cocci Time to result
A

: 2 - 8 hours

29
Q
  • Automated Microbiology Bacterial Identification and
    antibiotic susceptibility system.
A

VITEK

29
Q
  • kits include strips that contain up to 20 miniature
    biochemical tests.
A

API (ANALYTICAL PROFILE INDEX)

29
Q

negative oxidase test?

A

Escherichia, Klebsiella, Enterobacter, Serratia

30
Q

Vitek cards contains __ with nutrients and biochemical
test

A

64 wells

30
Q

VITEK principle?

A

Colorimetry

31
Q

Yeast like organisms: Time to result:

A

18 hours

32
Q

Gran negative bacilli: Time to result:

A

2 - 10 hours

33
Q

Gram positive spore forming bacilli: Time to result:

A

14 hours

33
Q
  • Anaerobic Bacteria Time to result:
A

6 hours

34
Q

Coryneform bacteria Time to result

A

8 hours

bacteriology (24 decks)

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