Agars Flashcards
Differentiation using hemolytic properties
BAP
BAP component?
Tryptic Soy Agar + 5% Sheep Blood/ 5% Horse Blood
- used to better isolate Streptococcus pneumoniae.
Gentamicin Blood Agar (GBA)
GBA component?
TSA+ 5% sheep blood +gentamicin
Differential of MAC
Fermentation of lactose
antibiotic inhibitor used to better isolate
Haemophilus spp.
Bacitracin-
Makes use of horse blood (High concentration of X and V
Factor)
Bacitracin Blood Agar (BCA)
MAC contains what to inhibit growth of gram pos bacteria?
Bile salts and crystal violet
V factor
NAD or NADP
CAP supplement?
VItalex
X factor?
Hemin
H2S indicator of SSA?
Ferric citrate
indicator of SSA?
Neutral red
Indicator of Mac Conkey agar?
neutral red
A modified deoxycholate agar
Salmonella shigella agar
Inhibitor of SSA
Bile salts, sodium citrate and brillant green
-Broth for the Isolation of Shigella and Salmonella
. SELENITE F broth
Medium for urine specimens?
BAP and MAC
-Strictly anaerobic bacteria (ie, those that cannot grow in the
presence of oxygen) grow at the bottom of the broth.
FLUID THIOGLYCOLLATE (FT)
Inhibitors of Thiosulfate citrate Bile Salt sucrose Agar?
Bile salt, Sodium cholate, alkaline pH
Agar selective for vibrio spp
TCBS agar
- Used for antibiotic susceptibility testing (Disk Diffusion,
test, Modified Hodge test, etc.) of most non-fastidious
bacteria.
Mueller Hinton Agat
Standard depth of MHA?
4mm
pH pf MHA?
7.2-7.4
Indicator of TCBS agar?
Bromthymol blue
-General purpose medium that is routinely used to grow
bacteria which tend to have high nutritional requirements
(ce, they are fastidious)
TRYPTIC SOY BROTH (TSB)
broth medium for the enrichment of Vibrio species from
food, water, and clinical samples.
ALKALINE PEPTONE WATER
- Used for antibiotic susceptibility testing of Haemophilus
species.
HAEMOPHILUS TEST MEDIUM (HTM)
Medium for stool
MAC, SSA,TCBS, Selenite F, APW,
-This broth supports the growth of anaerobes, aerobes,
microaerophilic, and fastidious microorganisms.
FLUID THIOGLYCOLLATE (FT)
Strict aerobic bacteria (ie, require oxygen for growth), such
as Pseudomonas sp., tend to grow toward the surface of the
broth
FLUID THIOGLYCOLLATE (FT)
-inhibitory for coliforms and certain other microbial specs,
such as focal streptococci, present in fecal specimens
SELENITE F Broth
- Culture broth to grow aerobic and facultative anaerobic
bacteria.
TRYPTIC SOY BROTH (TSB)
alkaline pH of this medium allows the growth of Vibrio
organisms while inhibiting the growth of commensal
intestinal bacteria.
ALKALINE PEPTONE WATER
medium for exudates?
BAP, MAC, FT
SIM: + for sulfide?
Black coloration
Medium for transudates?
BAP, CAP, MAC, TSB
Mediums for Respiratory specimens?
BAP, GBA,BCA,MAC
Triple Sugar Iron Agar (TSIA) compostion
0.1% glucose, 1% sucrose, 1% lactose
SIM; + for indole?
Reddish pink ring at junction
Indicator of TSIA?
Phenol Red
A/A?
Serratia
Escherichia
Enterobacter
Klebsiella
SIM rgt?
Kovac’s reagent (para-dimethylaminobenzaldehyde)
TSIA H2S indicator?
Ferrous sulfate
K/A:
Shigella
Yersinia
Citrobacter
Providencia
Proteus
Yersinia
A/A H2S+?
Citrobacter
Arizona
Proteus
K/A H2S+
Citrobacter
Arizona
Salmonella
Edwardsiella
Tests H2S production, indole reaction, and motility.
SIM (SULFIDE INDOLE MOTILITY)
K/K
Pseudomonas
Acinetobacter
SIM: + for motility?
spread out/ movement away from the stab line/
hazy appearance
used to determine the ability of an organism to utilize
sodium citrate as its only carbon source and inorganic
ammonium salts as its only nitrogen source
CITRATE
Citrate slant: Carbon source?
Sodium citrate
Citrate slant: nitrogen source?
inorganic Ammonium salts
pH indicator of citrate?
Bromthymol blue
+ result for citrate?
green to blue slant
- used to determine whether a gram negative rod
decarboxylates or deaminates lysine and forms H2S
Lysine Iron Agar
pH indicator of LIA?
Bromcresol Purple
Components of LIA?
Lysine, peptones, glucose, ferric ammonium
citrate and sodium thiosulfate
Positive result for oxidase test?
Purple
LIA decarboxylation?
Butt
LIA Deamination?
Slant
-Tests a microorganisms ability of hydrolyzing urea to
produce ammonia and carbon dioxide
Urease test
API (ANALYTICAL PROFILE INDEX) principle?
Turbidimetry
Urease test + color?
Pink color change
-To differentiate Enterobacteriaceae from
non- Enterobacteriaceae
Oxidase test
-determine the sensitivity or resistance of pathogenic aerobic
and facultative anaerobic bacteria to various antimicrobial
compounds.
KIRBY-BAUER DISK DIFFUSION
Oxidase test detects the presence of?
Cytochrome oxidase
- Gram positive cocci Time to result
: 2 - 8 hours
- Automated Microbiology Bacterial Identification and
antibiotic susceptibility system.
VITEK
- kits include strips that contain up to 20 miniature
biochemical tests.
API (ANALYTICAL PROFILE INDEX)
negative oxidase test?
Escherichia, Klebsiella, Enterobacter, Serratia
Vitek cards contains __ with nutrients and biochemical
test
64 wells
VITEK principle?
Colorimetry
Yeast like organisms: Time to result:
18 hours
Gran negative bacilli: Time to result:
2 - 10 hours
Gram positive spore forming bacilli: Time to result:
14 hours
- Anaerobic Bacteria Time to result:
6 hours
Coryneform bacteria Time to result
8 hours