Respiration/ATP A2 Flashcards

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1
Q

ATP ->

A

ADP + Pi

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2
Q

why is ATP useful

A
released in small suitable amounts
broken down in one step
makes energy available rapidly
makes substances more reactive/lower activation energy by phosphorylating 
can be reformed
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3
Q

why is respiration affected by temperature

A

enzymes can be affected

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4
Q

some things we use ATP for

A

active transport
metabolic processes
movement

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5
Q

why is ATP made in respiration as well as photosynthesis

A

can’t always photosynthesis e.g. in the dark
some tissues cant photosynthesise
ATP can’t move between cells or stored
plants use more ATP than produced in photosynthesis

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6
Q

what happens in oxidation

A

lose electrons, lose H, gain O2

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7
Q

what happens in reduction

A

gain electrons, gain H, lose O2

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8
Q

where does glycolysis take place

A

cytoplasm

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9
Q

steps in glycolysis

A

Pi added to glucose (using 2 ATP)
glucose splits into 2 triose phosphate (3C)
TP oxidised NAD reduced
TP made into pyruvate releasing 2ATP

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10
Q

what molecules are made in glycolysis

A

ATP NADH and pyruvate

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11
Q

what molecules are needed for glycolysis

A

ATP, glucose, and NAD

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12
Q

where does link reaction take place

A

matrix of mitochondria

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13
Q

steps in link reaction

A

pyruvate is oxidised NAD reduced
pyruvate loses CO2 becomes 2C acetyl group
2C acetate joins coenzyme A makes acetyl coenzyme A

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14
Q

products from link reaction

A

CO2 NADH, acetyl coenzyme A

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15
Q

where does krebs cycle take place

A

matrix of mitochondria

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16
Q

steps of krebs cycle

A

2C acetyl coenzyme A joins with 4C molecule
NAD and FAD are reduced
substrate level phosphorylation
CO2 released

17
Q

products from the krebs cycle

A

ATP, NADH, FADH, CO2 4C molecule

18
Q

at what stage in respiration do breakdown products of lipids and amino acids enter

A

krebs cycle

19
Q

where does the electron transport chain take place

A

the inner membrane of mitochondria

20
Q

steps of electron transport chain

A

NADH and FADH release their H
this splits to protons and electrons
electrons move along carriers in ETC using redox reactions
releases free energy
protein channels use energy to actively transport H ions into intermembrane sapce
this diffuse down concentration gradient through ATP synthase into matrix
release ATP
in oxidative phophorylation
protons and electrons combine with O2 to make water

21
Q

what does O2 act as in electron transport chain

A

terminal electron acceptor

22
Q

products of electron transport chain

A

ATP, water, NAD, FAD

23
Q

molecules used in electron transport chain

A

NADH, FADH, ATP, O2

24
Q

colour change when methylene blue when it is reduced

A

blue to colourless

25
Q

what does anaerobic mean

A

without O2

26
Q

difference between anaerobic and aerobic respiration

A

NAD is regenerated/pyruvate removed. So glycolysis continues

27
Q

equation for anaerobic respiration in plants/microorganisms

A

pyruvate + reduced NAD -> ethanol + CO2 + NAD

28
Q

why can’t scientists use glucose when investigating the stages of respiration individually

A

because glucose cannot cross the mitochondrial membrane (it is normally broken down using glycolysis)

29
Q

what is the equipment used to measure rate of gas exchange

A

manometer

30
Q

how does a manometer work

A

CO2 absorbed by potassium hydroxide
O2 used
pressure decreases
level of liquid moves

31
Q

why would you leave a manometer for a while after setting it up?

A

allow equilibrium to be reached
allow for expansion/pressure change in apparatus
allow respiration rate of seeds to stabilise

32
Q

what pieces of information would you need to calculate rate of respiration

A

volume of tube
distance moved
time taken
mass of respiring organisms