Genetic Fingerprinting A2

Question 1

process of genetic fingerprinting

Answer 1

cut DNA using restriction enzyme
use electrophoresis to separate DNA fragments according to size
southern blotting/transfer to membrane
make single stranded
apply probe radioactively labelled
reference to tandem repeats
identify using autoradiography

Question 2

process of electrophoresis

Answer 2

move towards anode because charged

separates according to size/charge

Question 3

features that separate proteins by electrophoresis

Answer 3

mass
number of amino acids/polypeptides
charge
R group