Required Pratical-The Effect Of Ph Of Rate Of Reaction

Question 1

Step 1

Answer 1

Use the syringe to place 2cm^3 of amylase solution into a test tube

Question 2

Step 2

Answer 2

Use another syringe to add 1cm^3 of ph buffer solution to the test tube

Question 3

Step 3

Answer 3

Place the test tube into a water bath set at 30 degrees and leave for 5 minutes

Question 4

Step 4

Answer 4

Whilst waiting, add a drop iodine solution into each dimple of a spotting tile

Question 5

Step 5

Answer 5

After 5 minutes, use another syringe to add 2cm^3 of starch to the amylase/buffer solution, start stop clock and leave it on throughout the test. Mix using a plastic pipette

Question 6

Step 6

Answer 6

Remove a drop if amylase/starch/buffer mixture after 30 seconds and add to the first drop of iodine on your spotting tile

Question 7

Step 7

Answer 7

Wait another 30 seconds. Then remove a second drop of the mixture to add to the next drop if iodine

Question 8

Step 8

Answer 8

Repeat step 6 until the iodine solution and the amylase/buffer/starch mixture remains orange.

Question 9

Step 9

Answer 9

Record the time taken for the amylase to fully digest the starch
.

Question 10

Step 10

Answer 10

Repeat the whole procedure with a different ph buffer