required practical 1 Flashcards
independant variable
temperature of the water bath ( 20,40,60)
dependant variable
time taken for the X to become visible
control variable
milk being at 37 degrees
volume of milk and its pH
temperature of the solution
how are the control varriables controlled
using at 7pH buffer
syringe to make 3% milk concentrartion and 0.5% trypsin
syringe to measure 2 cm3 of trypsin and 10 cm3 of powdered milk solution
errors that could occur
not starting the timer quick enough
not resting the timer
limitations
water bath isnt thermostatically controlled so it isnt reliable
not using a pH buffer
improvements
use thermostatically controlled water bath
use a pH buffer
what is RP1
rate of enzyme controlled reactions for the cloudy solution to become colourless