RED BLOOD CELL SUSPENSION

Question 1

test we need rcs

Answer 1

forward typing using tube method
crossmatching
detector of secretor status
rbc phenotyping

Question 2

how many tubes are needed for rcs

Answer 2

4 tubes

plasma
washed cells
5% rbc suspension
2% red blood cell suspension

Question 3

_______is a critical step in any laboratory procedure and should be done carefully

Answer 3

Labeling

Question 4

clerical error encounter in making rcs

Answer 4

mislabeling

Question 5

after labeling, what’s the next step

Answer 5

centrifuge

Question 6

preferred anticoagulant during preparation of red cell suspension

Answer 6

EDTA

Question 7

use of separating plasma

Answer 7

for future test

Question 8

note after removing plasma and transferring of washed rbc

Answer 8

This tube should be no more than half
full of cells.

Question 9

note in adding NSS to the washed cells

Answer 9

Be careful not to contaminate the tip of
the wash bottle dispenser with blood.

Question 10

we Add NSS from the wash bottle to the “WashedCells” tube until the liquid level is about_________” from the
top

Answer 10

½

Question 11

speed of centrifuge every time we spin it after washing

Answer 11

3400 rpm for 1 mn

Question 12

: Never open the lid of the centrifuge before the spinning
action has come to a complete stop

true or false

Answer 12

true

Question 13

why do we bound red cells

Answer 13

to remove unbound antibodies

Question 14

in blood bank, how many times we washed red cells

Answer 14

3-4 times

Question 15

common percentage of rcs

Answer 15

2-5%

Question 16

unwashed rbc will lead to

Answer 16

false negative result

Question 17

end color of RCS

Answer 17

tomato red appearance