IMMUNOHEMATOLOGIC REACTIONS Flashcards
in crossmatching, the positive reaction signifies
hemolysis and agglutination
In pre analytical phase, The type of blood collecting tube/s to be used depend/s on the ________ requested by the
physician
test/s
In pre-analytical phase, upon receiving the properly coagulated specimen of adequate volume of blood sample in the laboratory. What are the things that the medtech must do and consider
check for proper labeling
duly log the information into the laboratory information system or its alternative
materials and devices appropriate for the test procedure are then prepared
if there’s an agglutination and hemolysis reaction in crossmatching, the result means
incompatible
if there’s no agglutination and hemolysis reaction in crossmatching, the result means
COMPATIBLE
siginificance of agglutination in routine blood bag
we must be able to check it as well microscopically
2 step of agglutination
sensitization
lattice formation
best Ig for agglutinationn
IgM
steps in slide method agglutination
divide the slide into to (unknown U and negative control NC)
add 1 drop anti D antisera in unknown
add 1 drop of nss in negative control
add 1 drop of RCS each tube
mix the reagent and the blood sample using an applicator stick
add coverslip in negative control
wait for 2 minutes
interpret the result under the microscope in LPO objective
combination of agglutinated and non-agglutinated cells
mixed field
how do we interpret mixed field?
consider it positive
how do we interpret rouleaux formation?
stack of coins
false positive result - pseudoagglutination
what type of abo group discrepancies do rouleaux formation do belong?
group 3 abo discrepancies
rouleaux formation of red blood cells are seen in a patient with a condition associated with IgG, which condition is it?
multiple myeloma
destruction of rbc
hemolysis
classification of hemolysis
no hemolysis
partial hemolysis
complete hemolysis
steps in hemolysis experiment
prep 2 test tubes (unknown, Neg)
place 2 drops of 5% RCS in each tube
place 2 drop of distilled water in unknown
place 2 drop of nss in Neg
mix and parafilm
centri 15 seconds, 3400 rpm
do not dislodge and interpret the result
intact cell button, clear supernatant
___ hemolysis
no hemolysis
presence of cell button, pink supernatant
__ hemolysis
partial hemolysis
absence of cell button, red supernatant
__ hemolysis
complete hemolysis
speed of centri for hemolysis experimentation
3400 rpm for 15 seconds
