ABO B.TYPING - TUBE METHOD

Question 1

reagents used in FORWARD ABO TYPING TUBE METHOD

Answer 1

*Typing Sera A (Anti-A Antisera)
*Typing Sera B (Anti-B Antisera)
*Group “O” Type Serum for tube ANTI-AB

Question 2

__ are the most frequent errors
in the blood bank.

Answer 2

clerical errors

Question 3

how many drops of reagent is used for forward typing tube method

Answer 3

2 drops of anti sera

Question 4

how many drop of RCS is used

Answer 4

one drop 2% to 5%

Question 5

rpm and time of centrifuge of FORWARD TYPING TUBE METHOD

Answer 5

3400 rpm for 15 seconds

Question 6

what does the manner of reporting for tube method for ABO stands for

Answer 6

4+ one solid clump
3+ several large clump
2+ numerous smaller clump
1+ barely discernible clumps

Question 7

what are we observing for forward typing tube method to interpret the result

Answer 7

hemolysis or agglutination

Question 8

serves as our control for FORWARD TUBE MTHOD

Answer 8

anti-AB tube,

Question 9

blood group that will react positively to anti-ab tube or control of forward tube method

Answer 9

blood group A
blood group B
blood group AB

Question 10

In forward typing tube method

Observe suspected weakly reacting results by viewing the mixture under ____
objective of the compound microscope

Answer 10

low power

Question 11

reagents used in reverse blood typing - tube method

Answer 11

known a and b cells

Question 12

specimen used in reverse blood typing - tube method

Answer 12

patient’s serum or plasma

Question 13

Detects ABO antibodies in the patient’s serum by using known reagent
RBC’s (A and B cells)

Answer 13

reverse blood typing

Question 14

Only unique to the ABO blood group system; checks results of forward
typing

Answer 14

reverse blood typing

Question 15

abo antibodies are not normally present at birth, if present at birth, they originated from the mother through

Answer 15

placental leakage during delivery

Question 16

ABO antibodies develop ___ months after birth

Answer 16

3-6 months

Question 17

ABO antibodies are ___ in nature

Answer 17

Predominantly IgM and react at room temperature or colder

Question 18

ABO antibodies occur in two forms such as

Answer 18

naturally occuring antibodies
immune antibodies produced during incompatible transfusion or incompatible pregnancies

Question 19

how many drops of serum is added into 4 tubes prepared for reverse typing of ABO

Answer 19

2 drops each serum

Question 20

centri time and speed for reverse typing abo blood group

Answer 20

15 seconds at 3400 rpm