DETERMINATION OF SECRETOR STATUS

Question 1

If an individual has the secretor gene (SeSe or Sese), ABH
soluble antigen will be present and can be detected on the
following secretions.

Answer 1

Saliva, Tears, Urine, Digestive juices, Bile, Milk, Amniotic
Fluid, Pathologic fluids such as peritoneal, pericardial,
ovarian cyst

Question 2

specimen for determination of secretor status

Answer 2

saliva

Question 3

principle of determination of secretor status

Answer 3

hemagglutination inhibition

Question 4

It is based on the idea that patients ABH soluble antigen in saliva will be neutralized by
antibody reagent and an indicator cell is added

Answer 4

hemagglutination Inhibition

Question 5

Positive in hemagglutination inhibition

Answer 5

no agglutination - presence of ABH soluble antigen in the saliva

Question 6

negative in hemagglutination inhibition

Answer 6

with agglutination - absence of ABH soluble antigen in the saliva

Question 7

indicator cells used in determination of secretor status - hemagglutination inhibition

Answer 7

known cells

Question 8

Chew a piece of ___ to stimulate secretion of saliva

Answer 8

paraffin wax

Question 9

how many ml of saliva we must collect

Answer 9

2-3 ml of saliva

Question 10

we place the saliva in a boiling water for how many minutes right after collection

Answer 10

10mns

Question 11

purpose of boiling saliva for 10 mns

Answer 11

this inactivates enzymes that might otherwise destroy blood group substances

Question 12

how many mins we need to centi the saliva after boiling

Answer 12

3400 rpm for 10 mns

Question 13

after centri, we are collecting supernatant with a physical characteristic of

Answer 13

clear or slightly opalescent.

Question 14

the saliva (supernatant) must be initially diluted with ___ of a ratio of ___

Answer 14

NSS ratio: 1:2

Question 15

why do we need to dilute the saliva with NSS

Answer 15

undiluted saliva contains nonspecific glycoproteins that can
INHIBITS antisera and lead to incorrect results

Question 16

dilution of saliva with NSS is done by

Answer 16

This can be done by mixing 1mL of supernatant (saliva) and 1mL of NSS

Question 17

the reagent we need for determination of the status is ___

Answer 17

1:4 dilution of anti-A and Anti B antisera

Question 18

the 1:4 dilution of anti-A and Anti B antisera is can be done by

Answer 18

1 ml of antisera mixing by 3 ml of NSS

Question 19

do we have to mix the diluted saliva once added to the diluted anti sera

Answer 19

no, we just need to observe how they both combine and made a suspension

Question 20

after mixing the diluted saliva and diluted anti sera, we need to incubate all tubes at room temperature for how many mns

Answer 20

10 mns