Quiz 3.3 departs

Question 1

Characte of replication

Answer 1

Demiconservative
Bidirectional
Semicontinuous

Question 2

The eye/bubble is found where

Answer 2

where there is more A-T bonds

Question 3

ATPs used per base pair unwinded

Answer 3

2

Question 4

Forces the strands apart, anti-double helix

Answer 4

Helicase

Question 5

Prevent reannealing of strands

Answer 5

SSB/HD

Question 6

Reversible cut a single strand of the double helix

Answer 6

Type I DNA topoisomerase/DNA swivelase

Question 7

bind tightly to both strands of the DNA and make attansient break in the strands, introduces negative supertwists

Answer 7

Type 2 DNA topoisomerase

Question 8

Discontinuous fragments during the action of DNA polymerase

Answer 8

Okazaki

Question 9

Only prokaryotic polymerase isoform na may 5-3 exonuclease activity

Answer 9

Pol I

Question 10

Daughter starnd that is synthesiEd in 5-3 continuously

Answer 10

leading

Question 11

Daughter strand synthesized in the 5-3 direction discontinuosly

Answer 11

Lagging

Question 12

Fragments are joined together by ___ to form one daugter strand

Answer 12

DNA ligase

Question 13

Two enzymes that synthesize the primer

Answer 13

Rna polymerase for leading

primase (from G gene) for lagging

Question 14

The building blocks for rna primer and the product release with each phosphodiester bond formed

Answer 14

5’ ribonucleoside triphosphate

PPi

Question 15

prepriming complex assembled that binds to the single stranded dna displacing some ssDNA (protein + primase complex)

Answer 15

Primosome

Question 16

Chain elongation is catalyzed by

Answer 16

Pol 3

Question 17

Nucleotide uikding blocks for chin elongation

Answer 17

5’ deoxyribonucleoside triphosphate

Question 18

Inhibits pol alpha delta epsilon

Answer 18

Aphidicolin

Question 19

dideoxyNTPs inhibit what

Answer 19

B G strrongly

D E weakly

Question 20

n-ethylmaleimide inhibits

Answer 20

A G D E

Question 21

Animal dna polymerase: with primase activity, lagging strand replicase

Answer 21

Alpha

Question 22

Animal dna polymerase: for dna repair

Answer 22

Beta

Question 23

Animal dna polymerase: for mitochondrial dna repli

Answer 23

Gamma

Question 24

Animal dna polymerase: for proofreading (3-5 exonuclease), complexed with PCNA for u nlimited peovessivity

Answer 24

delta

Question 25

Animal dna polymerase: highly processive even without pcna

Answer 25

Epsilon

Question 26

Anima dn apolym delta needs to complex with what for unli processivity

Answer 26

delta

Question 27

Saan sa cell cycle ung dna repli

Answer 27

S phase

Question 28

Action of Ara A and C antiviral drugs

Answer 28

Inhib dna repli bu acting as analogues

Question 29

This inhibitor insert itself with dna inhibiting helicase Something acridine orange

Answer 29

Actinomycin D

Question 30

Inhiitor of dna gyrase

Answer 30

Novobiocin Oxalinic acid Nalidixic acid Coumermycon

Question 31

Responsible for dna directed rna synth

Answer 31

Rna polymerase

Question 32

Requirmeent aof rna polymerase

Answer 32

noncoding strand | Ribonucleoside triphosphates for substrate

Question 33

The transcription unit extends fron where to where

Answer 33

promoter region to terminator region

Question 34

Kind of termination where Tus is dislodged

Answer 34

Permissive

Question 35

Kind of termination where Tus-Ter is still intact

Answer 35

nonpermissive

Question 36

Subunits of the e coli rna polymease

Answer 36

2 alpha 1 beta 1 beta prime 1 sigma

Question 37

Stages of replication

Answer 37

``` Prepriming Polymerization Inititation Elongation Removal of primer Dna ligas ```

Question 38

Specific regions contained in dna recognized by the rna polymerase

Answer 38

Promoter region

Question 39

Which subunit in rna polymerase recognizes the promoter region

Answer 39

sigma

Question 40

Sequence of 7 nucleotides within promoter region Orients rna ploymerase as to the direction and start of synthesis Located 5-10 bases fron the first base to eb copied

Answer 40

Pribnow box

Question 41

sequce of pribnow box

Answer 41

TATAAT

Question 42

Sequence of the 35 sequence

Answer 42

TTGACA

Question 43

Initial site of sigma binding

Answer 43

35 sequence

Question 44

Causes localized melting of dna about 10 bases from probnow box

Answer 44

open promoter region

Question 45

Two binding sites of rna polymerase

Answer 45

inittitation site and elongation site

Question 46

The initiation site of rna polym binds only what

Answer 46

Purine ntps

Question 47

Binds to beta subunit if rna polymerase preventing incoming ntps from binding

Answer 47

Rifamycin SV

Question 48

Allows first dinucleotide to form but boocks movement of polymerase along the dna

Answer 48

Rifampicin

Question 49

Elongation (transcription) is cRried our by

Answer 49

Core enzyme

Question 50

The sigma unit dissociates when

Answer 50

8 bases polymerized

Question 51

Special termination protein

Answer 51

rho-p?

Question 52

How does rho terminate transcription

Answer 52

Finds bubble and unwinds it to release the rna chain

Question 53

Termination sites can be dound where

Answer 53

sequence of high GC near loop Sequence of As in the dna Inverted repeat base sequence with a central nonrepeating segment

Question 54

Rna polymerase species: synthesizes the large ribosomal RNAs in the nucleolus

Answer 54

1

Question 55

Rna polymerase species: produces small RNAs including tRNA and small 5S ribosomal RNA Less sensitive to inhibition by alpha-amanitin

Answer 55

3

Question 56

Rna polymerase species: synthesize the precursors of mRNA

Answer 56

2

Question 57

Consensus sequences similar to the pribnow box

Answer 57

TATA | CAAT

Question 58

recognition sites in eikaryotic promoters

Answer 58

Tata or caat box

Question 59

Inhibits rna polymerase 2, a potent toxin produced by a poisonous mushroom

Answer 59

alpha amanitin