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MedBiochem > Quiz 3.3 departs > Flashcards

Quiz 3.3 departs Flashcards

1
Q

Characte of replication

A

Demiconservative
Bidirectional
Semicontinuous

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2
Q

The eye/bubble is found where

A

where there is more A-T bonds

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3
Q

ATPs used per base pair unwinded

A

2

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4
Q

Forces the strands apart, anti-double helix

A

Helicase

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5
Q

Prevent reannealing of strands

A

SSB/HD

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6
Q

Reversible cut a single strand of the double helix

A

Type I DNA topoisomerase/DNA swivelase

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7
Q

bind tightly to both strands of the DNA and make attansient break in the strands, introduces negative supertwists

A

Type 2 DNA topoisomerase

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8
Q

Discontinuous fragments during the action of DNA polymerase

A

Okazaki

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9
Q

Only prokaryotic polymerase isoform na may 5-3 exonuclease activity

A

Pol I

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10
Q

Daughter starnd that is synthesiEd in 5-3 continuously

A

leading

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11
Q

Daughter strand synthesized in the 5-3 direction discontinuosly

A

Lagging

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12
Q

Fragments are joined together by ___ to form one daugter strand

A

DNA ligase

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13
Q

Two enzymes that synthesize the primer

A

Rna polymerase for leading

primase (from G gene) for lagging

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14
Q

The building blocks for rna primer and the product release with each phosphodiester bond formed

A

5’ ribonucleoside triphosphate

PPi

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15
Q

prepriming complex assembled that binds to the single stranded dna displacing some ssDNA (protein + primase complex)

A

Primosome

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16
Q

Chain elongation is catalyzed by

A

Pol 3

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17
Q

Nucleotide uikding blocks for chin elongation

A

5’ deoxyribonucleoside triphosphate

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18
Q

Inhibits pol alpha delta epsilon

A

Aphidicolin

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19
Q

dideoxyNTPs inhibit what

A

B G strrongly

D E weakly

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20
Q

n-ethylmaleimide inhibits

A

A G D E

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21
Q

Animal dna polymerase: with primase activity, lagging strand replicase

A

Alpha

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22
Q

Animal dna polymerase: for dna repair

A

Beta

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23
Q

Animal dna polymerase: for mitochondrial dna repli

A

Gamma

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24
Q

Animal dna polymerase: for proofreading (3-5 exonuclease), complexed with PCNA for u nlimited peovessivity

25
Q

Animal dna polymerase: highly processive even without pcna

26
Q

Anima dn apolym delta needs to complex with what for unli processivity

27
Q

Saan sa cell cycle ung dna repli

28
Q

Action of Ara A and C antiviral drugs

A

Inhib dna repli bu acting as analogues

29
Q

This inhibitor insert itself with dna inhibiting helicase

Something acridine orange

A

Actinomycin D

30
Q

Inhiitor of dna gyrase

A

Novobiocin
Oxalinic acid
Nalidixic acid
Coumermycon

31
Q

Responsible for dna directed rna synth

A

Rna polymerase

32
Q

Requirmeent aof rna polymerase

A

noncoding strand

Ribonucleoside triphosphates for substrate

33
Q

The transcription unit extends fron where to where

A

promoter region to terminator region

34
Q

Kind of termination where Tus is dislodged

A

Permissive

35
Q

Kind of termination where Tus-Ter is still intact

A

nonpermissive

36
Q

Subunits of the e coli rna polymease

A

2 alpha
1 beta
1 beta prime
1 sigma

37
Q

Stages of replication

A 
Prepriming
Polymerization
Inititation
Elongation
Removal of primer
Dna ligas
38
Q

Specific regions contained in dna recognized by the rna polymerase

A

Promoter region

39
Q

Which subunit in rna polymerase recognizes the promoter region

40
Q

Sequence of 7 nucleotides within promoter region

Orients rna ploymerase as to the direction and start of synthesis

Located 5-10 bases fron the first base to eb copied

A

Pribnow box

41
Q

sequce of pribnow box

42
Q

Sequence of the 35 sequence

43
Q

Initial site of sigma binding

A

35 sequence

44
Q

Causes localized melting of dna about 10 bases from probnow box

A

open promoter region

45
Q

Two binding sites of rna polymerase

A

inittitation site and elongation site

46
Q

The initiation site of rna polym binds only what

A

Purine ntps

47
Q

Binds to beta subunit if rna polymerase preventing incoming ntps from binding

A

Rifamycin SV

48
Q

Allows first dinucleotide to form but boocks movement of polymerase along the dna

A

Rifampicin

49
Q

Elongation (transcription) is cRried our by

A

Core enzyme

50
Q

The sigma unit dissociates when

A

8 bases polymerized

51
Q

Special termination protein

52
Q

How does rho terminate transcription

A

Finds bubble and unwinds it to release the rna chain

53
Q

Termination sites can be dound where

A

sequence of high GC near loop
Sequence of As in the dna
Inverted repeat base sequence with a central nonrepeating segment

54
Q

Rna polymerase species: synthesizes the large ribosomal RNAs in the nucleolus

55
Q

Rna polymerase species: produces small RNAs including tRNA and small 5S ribosomal RNA

Less sensitive to inhibition by alpha-amanitin

56
Q

Rna polymerase species: synthesize the precursors of mRNA

57
Q

Consensus sequences similar to the pribnow box

58
Q

recognition sites in eikaryotic promoters

A

Tata or caat box

59
Q

Inhibits rna polymerase 2, a potent toxin produced by a poisonous mushroom

A

alpha amanitin

MedBiochem (16 decks)

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