quiz 2.3 AA metab Flashcards
average daily protein turnover
250g/day
cases na may positive nitrogen balance
growing child
pregnant
convalescing pipz
refeeding after starvation
AA that does not undergo transamination
lysine
threonine
coenzyme in transamination
pyridoxal phosphate
product after trnsamination
alpha ketoacid
ATP and GTP are the positive allosteric effectors for the formation of ____ while ADP and GDP are for ____
glutarate
alpha-ketoglutarate
the only dehydrogenase which is non-specific for NAD and NADP
glutarate dehydrogenase
oxidase primarily involved in the deamination of lysine
L-amino acid oxidase
asparaginase converts asparagine to
ammonia and aspartate
important in kidney which helps excrete H in the form of NH4
glutaminase
direct deamination of histidine by ___ and ____ produces ___
histidase/histidine ammonia lyase
urocanic acid
dehydrative deamination of serine and threonine prodcues ____ and ___ respectively
pyruvate
alpha-ketobutyrate
3 ways ammonia is disposed
alanine cycle
glutamine cycle
urea cycle
urea cycle aka
Krebs-Henseleit cycle
where does urea cycle occur
liver
saan galing ung two nitrogens sa urea
from NH3 thru glutamate dehydrogenase
from aspartate by transamination
rate limiting enyzme fi the urea cycle
carbamoyl phosphate synthase 1
formation of carbamoyl phosphate by CPS1 is driven by
cleavage of 2 mols of ATP
ornithine plus carbamoyl phosphate by ornithine transcabamoylase forms
citrulline
in the urea cycle, citrulline condenses with aspartate to form ____ catalyzed by ____
argininosuccinate
argininosuccinate synthetase
arginine is cleaved by arginase to form
ornithine and urea
argininosuccinate is cleaved by ___ to form
argininosuccinate lyase
arginine and fumarate
after arginine is cleaved to ornithine and urea, what are the fates of these two
urea - excreted trhu urine
ornithine - enters mitcochondria
what component in the urea cycle enters the TCA
fumarate
where urea increased, starvation and high protein diet or low protein diet
starvation and high protein diet
the allosteric activator of CPS1
N-acetylglutamate
positive allosteric activator of NAG synthetase
arginine
amino acids which, in the final step of its emtab, can be converted into glucose pyruvate or a TCA cyle intermediate that can be converted to OXAA
glucogenic
amino acids which can be converted into fat
ketgenic
what are the purely ketogenic AAs
leu
lys
what AAs are both keto and glucogenic
phe ile trp tyr thr
amino acids catabolized via pyruvate
alanine serine glycine cysteine & cystine threonine tryptophan hydroxyproline
in the metab of alanine, the end product ____ whose formation is catalyzed by _____ ultimately results to the formation of ____
pyruvate
alanine aminotransferase
OXAA
alanine: keto or gluco?
glucogenic
in the first step, serine undergoes ____ by ___ to form ____
dehydrative deamination
serine dehydrogenase
pyruvate
pyruvate from serine in its metab is the converted to ___ which is then subsequently oxidize to __ and ___
glycine
CO2 and ammonia
catalyzes the 2nd step in the metab of serine
serine hydroxymethyltransferase
metab of glycine
glycine > (serine hydroxymethyltranferase) > serine > (serine dehydrogenase) > pyruvate
product when glycine is acted upon by D-amino acid oxidase
glyoxylate
glycine from serine or from diet can also be oxidized by ___ to yield a second equivalent of ______
glycine cleavage complex
N5,N10-methylene THF
universal sulfur donor
3’-phosphoadenosine-5’-phosphosulfate (PAPS)
simplest path of cystine
catalyzed by liver desulfurase to produce pyruvate
cysteine > (_______) > cysteinesulfinate
cysteine dioxygenase
cysteine > (cystein dioxygenase) > ______________
ccysteinesulfinate
cysteinesulfinate undergoing transaminattion produces what b
beta-sulfinylpyruvate
cysteinesulfinate undergoing decarboxylation and oxidation produces
taurine
beta-sulfinylpryuvate undergoing desulfuration yields
bisulfite
bisulfite acted upon by sulfite oxidase yields
sulfate and H2O2
the sulfate yielded from bisulfate will be used to
make PAPS
cystine > (__________) > cysteine
NADH linked-cystine reductase
how many moles of L-cysteine formed from a cystine acted upon by cysteine reductase
2 moles
the enzyme ___ can transfer the sulfur from one cysteine to another to form _____ and _____-
cystathionase
thiocysteine & pyruvate
transamination of cysteine yields __________ which can react with sulfite to form ____ and __
beta-mercaptopyruvate/3-mercaptopyruvate
thiosulfate & pyruvate
thiosulfate and thiocysteine can detoxify cyanide by the action of ___ incorporating the sulfur into the cyanide
rhodanase
pathway ng propionylCoA to succinylCoA
propionylCoA > (carboxylation) > D-methylmalonyl CoA > (racemase) L-methylmalonyl CoA > (mutase) > succinyl CoA
threonine > (serine/threonine dehydrogenase) > _____
a-ketobutyrate
a-ketobutyrte > (a-ketoacid dehydrogenase) > _____
propionyl CoA
threonine > (threonine dehydrogenase) > ______
a-amino-b-ketobutyrate
pathway ng a-amino-b-ketobutyrate hanggang pyruvate pakielaborate nang maging hero na si marcos
a-amino-b-ketobutyrate > aminoacetone > methylglyoxal > pyruvate
threonine acted upon by threonine aldolase produces __ and __-
acetylCoA and pyruvate
first enzyme of the trp metab is ___ than opens the indole ring
iron porphyrin oxygenase
first key branch point of trp metab
kynurenine
3 possible products/intermediate after the first branch point of trp metab
kynurenate
anthranilate
3-hydroxykynurenine
which of the products from kynurenine is an anticonvulsant
kynurenate
a urinary metabolite from trp seen during its metab
xanthurenate
xanthurenate came directly from or is a product of what
transaminated 3-hydroxykynurenine
2nd key branch point in trp metab
2-amino-3-carboxymuconic semialdehyde
the roduct from trp metab that is the precursor of NAD and NADP
nicotinate mononucleotide
from the 2nd branching point of trp metab, what are the ultimate products
picolinate
nicotinate mononucleotide
glutarylCoA > acetylCoA
in benign hyperhydroxyprolinemia, alin ung wala or blcoked
hydroxyproline dehydrogenase
in type 2 hyperprolinemia, alin ung wala or blocked
2nd dehydrogenase rxn
ulitmate products of hydroxyproline metab
glyoxylate
pyruvate
amino acids catabolized by OXAA
aspartate
asparagine
aspartate + oxoglutarate > _______ + glutamate
OXAA
amino acids catabolized via a-KG
glutamine glutamate histidine proline arginine
convert glutamine to glutamate and NH3
glutaminase
where is glutaminase prominently found
kidney tuules
glutamate is converted to ____ by transamination or by oxidative deamination by glutamate dehyrdrogenase
a-KG
glutamate > (_______) > a-KG
glutamate drhydrogenase
what component in the gutamate dehydrogenase pathway is a substrate for urea cycle
NH4
ornithine in excess from the cleavage of arginine is transaminated to form
glutamate semialdehyde
can serve as a precursor for proline biosynthesis
glutamate dehydrogenase
common intermediate of proline and argnine
glutamate semialdehyde
end peoduct of his metab
glutamate
decarboxylation of histidine in the intest by nacte produces
histamine
the 1-carbon THF intermediate of histi metab
N5 formimino THF
intermediate in his metab tested in the skin
urocanate
urinary metabolite in the his metab
N-formiminoglutamate
what accumulates in histidinemia
imidazolepyruvate
imidazoleacetate
imidazolelactate
histidinemia produces what result in the ferric chloride test?
false pos
why false psoitive ang histidinemia sa ferric chloride
imidazolepyruvate
2 fates of phe metab
incorporation into polypeps
tyrosine
coenzyme required in the formation of tyrosine from phe
terrahydrobiopterin
the enzyme requiring tetrehydrobiopterin
phenylalanine hydroxylase
aside from the two main fates of phe in its metab, phe is also transaminated to form ____-, ______ or _____
phenyllactic acid
phenylacetic acid
glutamine
main pathway of tyosine degradation involce conversion to __ and ____
fumarate
acetoacetate
deficiency of phe hydroxylase
PKU
dse leads to painful corneal eruptions and photophobia
hypertyrosinemia
first genetic dse recognized
alkaptonuria
alkaptonuria is the defiency of
homogentisic acid oxidase
itsura ng urine sa alkaptonuria
black/darkening
characterisitic of type 1 tyrosinemia
cabbage-odor urine
deficiency of tyrosine transaminase
Richner-Hanhart syndrome (type2 tyrosinemia)
dse when tyrosine is not converted to thyroxine
cretinism
what is deificiecnt in type 1 tyrosinemia
fumarylacetoacetate hydrolase
which of the products of tyr metab is keto and which is gluco
fumarate - gluco
acetoacetate - keto
tyrpsine may serve as the precurosor of
catecholamines
universal emthyl donor
S-adenosylmethionine
SAM when devoid of methyl becomes
S-adenosylhomocysteine
path ng tyrosine to epineph
L-tyr > dopa > dopamine > norepi > epineph
what stimulates cystathione synthase
SAM
block of the rxn that converts homocysteine back to methione is what dse
type2 homocysteinuria
what conezyme does methionine synthase need
methylcobalamine
what enzyme convert homocysteine back to meth
methionine synthase
block of the rxn that uses cystathione synthase is what dse
type 1 homocysteinuria
cystathione = _____ + ______
homocysteine + serine
the intermediate comonly shared by meth and threo
a-ketobutyrate
what are the BCAAs
val
ile
leu
what is common in the metab of the the BCAAs
first 3 rxns
BCAA aminotansferase
BC a-ketoacid dehydrogenase
the next dehydrogenase
block in the 2nd step of BCAA metab causes
increases ketoacids and BCAAs
dse with burnt sugar odor, inc. BCAA, deficient BC a-ketoacid dehydrogenase
MSUD
which of the BCAA is purely keto, purely gluco and both
leu - keto
val - gluco
ile - both
intermediate from threonine before it ultimately becomes propionyl COA > succinyl CoA
a-ketobutyrate
dse malabsorption of trp, acuumulation of indicansa
blue diaper syndrome
amino acids cataolized into acetyl CoA and acetoacetate
trp
lys
leu
amino acids catabolized via succinylCoA
met
BCAAs
an intermediate in trp metab that antagonizes the effects of excfretory amino acids in teh brain
kynurenic acid
epsilon amino group of lysine is transferred to the a-keto carbon of a-KG forming the metabolite
saccharopine
the transmination of lysine is different from the others in a way that it
does not use PP
saccharopine is acted upon by __ to form __ and ___
aminoadipic semialdehyde synthase
glutamate
a-aminoadipic semialdehyde
ultimate end-product of lsine catab
acetoacetyl CoA
lysine is the precurosr of ___ required for the transport o FA into mitochondria for oxidation
carnitine
lysine + a-KG > (___) > saccharopine
lysine 2-oxoglutarate reductase-saccharopine dehydrogenase
intermediate commonly shared by trp and lys
a-ketoadipate
absence of the first enzyme in lys metab, ______, is the cause fo the dse _____-
lysine 2-oxoglutarate reductase-saccharopine dehydrogenase
hyperlysinemia
a dse of transport failure of dibasic AAs
familial lysinuria protein something
wat is decreased in familial lysinuria protein something
lys
arg
orni
path ng leu to acetylCoA and acetoacetate
leu > HMGCoA > acetylCoA + acetoacetate
end products of ile metab
succinylCoA
acetylCoA
AAs canno be synthesized sufficiently by the body
essential
the 3 essential AAs not related to lack of synthesis
arg
met
tyr
essential AAs
PVT TIM HALL
proline
valine
trp
thr
ile
met
his
arg
lys
leu
synthesis of nonessentials:
pyruvate > (ALT) > _____
alanine
metab of nonessentials:
OXAA > (aminoatransferase) > ___
aspartate
synth of noness AA
alanine + a-KG > (_____) > ____
glutamate pyruvate aminotransferase
glutamate
synth of noness AA
glutamate > (____) > _____
glutamine synthase
glutamine
synth of noness AA
aspartate > (___) > _____
asparagine synthase
asparagine
synth of noness AA
serine > (____) > _____
serine hudroxymethyl transferase
glycine
synth of noness AA
glycine aside from choline and serine can also be sunthesized from
gloxyli acid
synth of noness AA
possible sources/orig of glycine
serine
glyoxylic acid
chiline
synth of noness AA
glycine > (______) > ____ requiring the conezyme _____
hydroxymethyl transferase
serine
tetrahydrofolate
synth of noness AA
aside from glucine, serine may also be sunthesized from
3 phosphogluceric acid > phosphohusroxyphruvate > phosphoserine > serine
synth of noness AA
glutamate > ____ > delta1-pyrrolidine 5-carboxylic avid > ______
glutamate semialdehyde
proline
carbon skeleton of custeine is provided by
serine
sulfhydryl group in cysteine is provided by
homocyteine
deficiency or absence in meth synthase what daw
type 2 3 4 cystinuria
type 1 homocysteinurianis deficiency of
cystathione synthase
symptoms or signs in type1 gomocysteinuria
osteoporosis
mental retardation
another end peoduct from methionine during synth of cysteine
a-ketobutyrate
synth of noness AA
phe > (____) > ____
phe hudroxylase
tyr
converts dihydrobiopterin back to terrahydrobiopterin
dihydrobiopterin reductase
absence of phe hudroxylase
classic Pku
synth of noness AA
____ > (______) > arginine
argininosuccinate
argininosuccinase
continuous highexcretio of endogenous of oxalate and caox deposits in the kidney and urinary tract
primary hyperoxaluria
primary huperoxaluria is die to deficiency of
gloxylix acid oxidizig enzyme
glycine transaminase
defective syntheisjs of melanin
albinism
defiency in L methylmalonyl CoA mutase
methylmalonic aciduria
biosynthesis of catecholamines explain para mapatunayang nandaya ang liberal party nung election
tyr > (___) > dopa > (__) > dopamine > (___) > norepi > (___) > epineph
turosine hydroxylase
dopa carboxylase
dopamine B oxidSe
PNMT phenylethanolamine A methyl transferase
irinary metab in the catab of catechol and is the end product
vanillyl mandelate
urinary metabolite in the synthesis of melatonin
5-hudroxyindole 3avetate (5-HIAA)
sertoonin aka
5-huroxytryptamine
serotonin undergoing avetylation then methylation produces
melatonin
iodination of tyr produces
monoiodotyrosine
diiodotyrosine
coupled mono and diiodotyrosines are released as
thyroxine T4
ttriiodothyronine T3
decarboxylation of hisitidine
his > (____) > _____
hisitidine decarboxylase
histamine
3 shit used in the synth of creatinine
argi
gly
SAM
in creatinine synth
arg > (___) > ____
glycine cyclase transamidase
guanidoacetate
guanidoacetate when methylated becomes
creatine
creatine phosphate hs high every storage where
muscles
____ gives rise to a different melanin intermed of barying color and the black pigment ____
dopaquinone
eumelanin
derived from trp via anthranillic acid pathway
niacin
niacin is derived from trp via
anthranillic acid apthway
decarboxylation of glutamic acid forms
GABA
transamination of GABA produces
succnic acid semialdehyde
symth of polyamines
orni > (___) > _____ > (___)> _____ > ____
ODC pitrescine spermidine synthase spermidine spermi e
3 AAs used to synth gluta
glutamic acid
glycine
cysteine
ano ug path ng paggawa ng gluta
glutamate > (y-glutamate cysteine synthetase) > y-glutamylcysteine > (gluta synthase) > gluta
high levels of carbosine is found where
excitatroy tissues
decreased urinary 3methylhistidine what dae
wilson’s
devreased in wilson’s
3methylhisitidne
funcction of carbosine
avtivate myosin ATPase
^ iron uptake
structure of carnosine is ___ and answrine is _____
b-alamylhistidine
b-alamylmethylhistidine
sunthesized from arg ny an enzyme which uses NADPH as conezyme
NO
enzyem used to make NO
NOS
the hormone for regulation that is the only anabolic
insilin
purpose of T4 in hormone regulation
sensitization
counteregulatory hormones of insilin
glucagon epine cortisol somatos GH