QUIZ 3 Salivary Gland Development

Question 1

are salivary glands ectodermal or endodermal in origin?

Answer 1

• majority of people believe they are of ectodermal origin
• experiments in mice that study p63 genes provide evidence that they are of ectodermal origin

Question 2

what are the stages of salivary gland development?

Answer 2

Question 3

salivary gland development occurs mostly at the ___ stage

Answer 3

pre-natal

Question 4

if you combine salivary mesenchyme with mammary epithelium (nasal or pituitary), what happens?

Answer 4

salivary gland pattern with milk secretion

Question 5

if you combine salivary gland E13 (pseudoglandular) with mammary mesenchyme (mandibular arch limb bud), what happens?

Answer 5

failure to branch (aka failure to undergo morphogenesis)

Question 6

at what stages of gland development can you culture salivary gland cells?

Answer 6

• initial bud (12.5 weeks)
• pseudoglandular (13.5 weeks - best)
• you will NOT get growth if you try to culture cells from the prebud stage

Question 7

if you try to culture salivary epithelium alone, will it develop?

Answer 7

no

Question 8

what can you add to salivary epithelium for it to develop in culture?

Answer 8

matrigel or salivary mesenchyme

Question 9

___ has been used as the main model to study salivary gland differentiation

Answer 9

the mouse SMG

Question 10

salivary gland development involves the interaction of multiple ___ types

Answer 10

cell

Question 11

future studies are necessary to understand what 3 things in salivary gland development?

Answer 11

• what dictates the position of the glands
• how a cell becomes mucus or serous
• how individual cell types contribute to gland formation

Question 12

L1 peptide-conjugated fibrin hydrogels promote ___

Answer 12

salivary gland regeneration

Question 13

there is a rich level of active caspase-3 and survivin within the lumen of the salivary gland at the ___ stage

Answer 13

canalicular

Question 14

in the canalicular stage, terminal acinal cells have ___; this is how you can tell they are terminal cells

Answer 14

mucins

Question 15

___ levels are high in the terminal bud stage

Answer 15

EGF

Question 16

salivary glands continue developing in mice about how long after birth?

Answer 16

4 weeks

Question 17

which stage of human salivary gland development is depicted?

Answer 17

prebud stage

Question 18

which stage of human salivary gland development is depicted?

Answer 18

initial bud stage

Question 19

which stage of human salivary gland development is depicted?

Answer 19

canalicular stage

Question 20

describe afferent and efferent signals in salivary gland innervation

Answer 20

Question 21

when does the location of parasympathetic and sympathetic innervation develop in salivary glands?

Answer 21

• parasympathetic - at the same time as the gland
• sympathetic - post-natal (P3-P5)

Question 22

what is the parasympathetic neurotransmitter release in salivary glands?

Answer 22

acetylcholine is released either from the otic ganglion (innervates parotid) or from the submandibular ganglion (innervates the SMG and SL)

Question 23

what is the sympathetic neurotransmitter release of salivary glands?

Answer 23

noepinephrine is released by efferent nerves arising from the superior cervical ganglion away from the gland in the thoracic spinal cord

Question 24

during what stage of development can aplasia of lacrimal and salivary gland occur?

Answer 24

pre-bud to initial bud stage

Question 25

during what stage can lacrimoauriculodentodigital syndrome occur?

Answer 25

initial bud to pseudoglandular stage

Question 26

during what stage can hypohidrotic ectodermal displasia occur?

Answer 26

canalicular stage