quiz 14 Flashcards
Name the 2 main types of microscopes
Light and electron microscope
What does an electron microscope use to observe cells?
A beam of electrons
Which part of the microscope do we need to adjust to change the field of view?
The coarse focus wheel
What is a coverslip used for when viewing a slide under a microscope?
protect the objective lens and prevent the specimen from drying out
State the magnification equation
real size x magnification= image size
Give 2 disadvantages of using a light microscope compared to an electron microscope
lower magnification, lower resolution
Which objective lens should we always use when we start observing specimens using a microscope?
lowest power objective lens
Give 2 advantages of using a light microscope compared to an electron microscope?
can view live specimens and cheaper
What does a light microscope used to observe cells?
light
Before electron microscopes, why were many sub-cellular structures unknown to scientists?
They were too small to see
Why are most cells stained before being viewed under a microscope?
To add to contrast to sub-cellular structures can be seen within the cell
Which part of the microscope do we need to adjust to change the magnification?
the objective lens
Give 2 disadvantages of using an electron microscope compared to a light microscope
cannot view live specimen, more expenisve
Give 2 advantages of using an electron microscope compared to a light microscope
higher magnification, higher resultion
Define resultion or resolving power
the ability to distinguish 2 points as seperate