Protein Synthesis Flashcards
What is Protein Synthesis
Protein synthesis is used to make protein
What is the protein synthesis process
DNA Transcription mRNA Translation Protein
Transcription step
Isolate the gene
RNA polymearase unzips DNA. Start codon (AUG)
mRNA is created by RNA polymearase (U instead of T)
DNA rezipped
Caps are put on the ends, and splicing (Intron - Taken out, Exons - Glued)
Mature mRNA leaves the nucleus
How many nucleotides are needed to make an amino acids
3 nucleotides (triplets) = amino acids
What is splicing?
this is a process where some code in the nucleotide molecule is removed (called introns), while the rest (exons) are then joined together so there are no gaps.
What is mRNA?
messenger RNA
What is Translation
Process of mRNA to proteins
uses tRNA
What is tRNA
tRNA is a nucleic acid
Anti-codon (tRNA complimentary triplet)
Binds amino acids depending on mRNA codon
Translation Steps
Initiation
Elongates the chain of amino acids
Step 1 - Initiation
Ribosome binds to mRNA and tRNA starts anti codons
mRNA will move along Ribosome and matching tRNA anti codon will ‘deposit’ amino acid
Step 2 - Elongation
Elongation builds amino acids chain
mRNA move across, to allow the next codon to be ‘read’
Step 3 - Termination
Stop codon enters the ribosome and then the chain of A.A leaves the ribosome. Release factor is added to codon instead of a tRNA
How the protein functions
Before the protein can function it has to go through different steps
Folding into the correct shape
Processing (certain amount of aa)
Where it is located (moved to)
Combination (proteins can bond to be used for different things)