Protein Analysis Tools

Question 1

HCl Hydrolysis

Answer 1

Releases individual AAs
Gln–>Gla
Asn–>Asp
Trp is destroyed

Question 2

Cation exchange chromatography

Answer 2

AAs applied to a negative column
Weakly positive come off first
Strongly positive come off last

Question 3

Ninhydrin

Answer 3

Amount of AA proportional to concentration of purple color

Question 4

Edman degradation

Answer 4

Binds to free N-terminus

Can do up to 100AAs

Question 5

Trypsin cleavage

Answer 5

C-terminal side of Lys and Arg

Question 6

Chymotrypsin cleavage

Answer 6

C-terminal side of Trp, Tyr, Phe, Met, Leu

Question 7

Elastase cleavage

Answer 7

C-terminal side of Ala, Gly, Ser

Question 8

Chemical cleavage

Answer 8

CNBr breaks bond on C-terminal side of Met