Principles Of Immunodiagnostics Flashcards
What are the factors affecting measurement of antigen-antibody interactions?
Contrast affinity and avidity
What are the useful properties of monoclonal antibodies?
What are the useful properties of polyclonal antibodies?
What are immunoassays?
Explain the principle behind precipitation
What are the precipitation assays?
What are the factors affecting measurement of antigen-antibody reactions?
What is immunoelectrophoresis?
What are agglutination reactions?
What is direct agglutination?
What is passive agglutination?
What’s the principle behind hem agglutination?
What is hemagglutination inhibition?
What are the components of hemagglutination and hemagglutination inhibition ?
What are the limitations of agglutination?
Summarize ABO antibodies
Explain blood typing
Summarize blood type, blood antibodies and antigens
What does blood type look like?
What are immunoflourescent Assays?
Contrast direct and indirect immunofluorescent assays
What does immunofluorescent assays look like for antibodies ?
What are the limitations of immunofluorescence?
What is the principle of Enzyme-linked immunosorbant assay?
What are the types of ELISA?
What is the principle of western-blot analysis?
What does western blotting look like?
What are the advantages and limitations of western blot?
What are the main indirect methods of immunochromatography?
What is immune-chromatography?
What are the advantages and disadvantages of indirect methods of immuno-chromatography?
What is flow cytometry?
When do you use Ag vs Ab test?
What’s the impact of Sero-conversion?
Describe the generations of immunoassays and seroconversion
explain timing of antibody response in natural infection
