PRELIM LAB: MICROSCOPE

Question 1

The simplest type of
compound microscope

Answer 1

Brightfield compound
microscope.

Question 2

the word _______ refers to the fact that
magnified objects
appear as dark objects
against a bright
background.

Answer 2

“brightfield”

Question 3

The word “_________” from “Brightfield Compound Microscope” means that a specimen positioned properly on the stage of a microscope and illuminated by a light source will be magnified by TWO-LENS SYSTEM

Answer 3

compound

Question 4

The Path of LIght
Also known as the light source.

Answer 4

Iluminator

Question 5

Has lenses that direct the light rays through the specimen.

Answer 5

Condenser

Question 6

Light rays then pass into the objective lenses, the lenses closest to the specimen.

Answer 6

Objective lenses

Question 7

Then the image of the specimen is magnified again by the ocular
lens or eyepiece.

Answer 7

Ocular lens

Question 8

The oil used in oil immersion
is

Answer 8

CEDAR WOOD OIL

Question 9

CEDAR WOOD OIL REFRACTIVE INDEX

Answer 9

1.516.

Question 10

Remagnifies the image
formed by the objective
lens; standard magnifying
power is 10x.

Answer 10

Eyepiece/Ocular

Question 11

Holds the eyepieces in
place above the objective
lenses.

Answer 11

Eyepiece Tube

Question 12

Primary lenses that
magnifies the specimen.

Answer 12

Objective lenses

Question 13

A rotating turret that
houses the objectives.

Answer 13

Nosepiece

Question 14

Magnification: 4x
BAND: RED

Answer 14

SCANNER

Question 15

○ Magnification: 10x
BAND YELLOW
○ It usually forms the general outline or wider portion of the object.

Answer 15

LOW POWER OBJECTIVE (LPO)

Question 16

○ Magnification: 45x
BAND: BLUE
○ It is longer than the LPO and it forms a bigger image if the object in focus. In most cases, it is used to enlarge specimens that are so small under LPO.

Answer 16

HIGH POWER OBJECTIVE (HPO)

Question 17

○ Magnification: 100x
BAND: WHITE
○ HIGHEST DEGREE OF MAGNIFICATION
○ It is used to examine stained smear preparations of microorganisms using
immersion oil as their medium.

Answer 17

OIL IMMERSION OBJECTIVE (OIO)

Question 18

A bigger wheel used to
adjust the LPO in focusing; used also for INITIAL FOCUSING

Answer 18

Coarse Adjustment Knob

Question 19

A smaller wheel for FINAL FOCUSING of the specimen using HPO and OIO; used
also to make the specimen more vivid.

Answer 19

Fine Adjustment Knob

Question 20

Where the slide/specimen
is placed for focusing.

Answer 20

Stage

Question 21

It is used to hold the slide in place.

Answer 21

Stage Clips

Question 22

The light source of the
microscope.

Answer 22

Iluminator

Question 23

It is used to COLLECT and FOCUS the light from the illuminator on the specimen.

Answer 23

Condenser

Question 24

It CONTROLS THE AMOUNT of light reaching the specimen.

Answer 24

Iris Diaphragm

Question 25

Moves the condenser up or down to control the lighting focus on the specimen.

Answer 25

Condenser Focus Knob

Question 26

Calculating the Total Magnification

Answer 26

TOTAL MAGNIFICATION = Magnifying power of the eyepiece x Magnifying power of the objective used