PRELIM LAB: MICROSCOPE Flashcards
The simplest type of
compound microscope
Brightfield compound
microscope.
the word _______ refers to the fact that
magnified objects
appear as dark objects
against a bright
background.
“brightfield”
The word “_________” from “Brightfield Compound Microscope” means that a specimen positioned properly on the stage of a microscope and illuminated by a light source will be magnified by TWO-LENS SYSTEM
compound
The Path of LIght
Also known as the light source.
Iluminator
Has lenses that direct the light rays through the specimen.
Condenser
Light rays then pass into the objective lenses, the lenses closest to the specimen.
Objective lenses
Then the image of the specimen is magnified again by the ocular
lens or eyepiece.
Ocular lens
The oil used in oil immersion
is
CEDAR WOOD OIL
CEDAR WOOD OIL REFRACTIVE INDEX
1.516.
Remagnifies the image
formed by the objective
lens; standard magnifying
power is 10x.
Eyepiece/Ocular
Holds the eyepieces in
place above the objective
lenses.
Eyepiece Tube
Primary lenses that
magnifies the specimen.
Objective lenses
A rotating turret that
houses the objectives.
Nosepiece
Magnification: 4x
BAND: RED
SCANNER
○ Magnification: 10x
BAND YELLOW
○ It usually forms the general outline or wider portion of the object.
LOW POWER OBJECTIVE (LPO)
○ Magnification: 45x
BAND: BLUE
○ It is longer than the LPO and it forms a bigger image if the object in focus. In most cases, it is used to enlarge specimens that are so small under LPO.
HIGH POWER OBJECTIVE (HPO)
○ Magnification: 100x
BAND: WHITE
○ HIGHEST DEGREE OF MAGNIFICATION
○ It is used to examine stained smear preparations of microorganisms using
immersion oil as their medium.
OIL IMMERSION OBJECTIVE (OIO)
A bigger wheel used to
adjust the LPO in focusing; used also for INITIAL FOCUSING
Coarse Adjustment Knob
A smaller wheel for FINAL FOCUSING of the specimen using HPO and OIO; used
also to make the specimen more vivid.
Fine Adjustment Knob
Where the slide/specimen
is placed for focusing.
Stage
It is used to hold the slide in place.
Stage Clips
The light source of the
microscope.
Iluminator
It is used to COLLECT and FOCUS the light from the illuminator on the specimen.
Condenser
It CONTROLS THE AMOUNT of light reaching the specimen.
Iris Diaphragm
Moves the condenser up or down to control the lighting focus on the specimen.
Condenser Focus Knob
Calculating the Total Magnification
TOTAL MAGNIFICATION = Magnifying power of the eyepiece x Magnifying power of the objective used
