MIDTERM LAB: VENIPUNCTURE Flashcards
act of drawing or removing blood from the circulatory system through a cut (incision) or puncture to obtain a sample for analysis and diagnosis
PHLEBOTOMY
PURPOSE OF PHLEBOTOMY:
- To obtain blood for diagnostic purposes.
- To monitor levels of blood components.
- To administer therapeutic treatments including medications, nutrition, or chemotherapy.
- To remove blood due to excess levels of iron or erythrocytes
- To collect blood for later uses, mainly transfusion either in the donor or in another person.
MATERIALS/EQUIPMENT:
- 21-gauge syringes, 2-way needle, & Feather lancet
- Tourniquet
- Wet and dry cotton balls
- 70% alcohol
- Micropore tape/plaster
- Evacuated Collection Tubes:
- Puncture-proof container for sharp objects
- Gloves, Mask, and Lab gown
- Marking pen (for labelling)
The essential elements of the requisition form are:
- Patient’s surname, first name, and middle initial.
- Patient’s ID number
- Patient’s DOB and sex.
- Requesting physician’s complete name.
- Source of specimen. This information must be given when requesting microbiology, cytology, fluid analysis, or other testing where analysis and reporting is site specific.
- Date and time of collection.
- Initials of phlebotomist.
- Indicating the test(s) requested
The key elements in labeling are:
- Patient’s surname, first, and middle
- Patient’s ID number
- Date and time of extraction and initials of the phlebotomist
The tubes are designed to fit with a predetermined volume of blood by vacuum. The rubber stoppers are color-coded according to the additive that the tube
contains. Various sizes are available. Blood should NEVER be poured from one tube to another
since the tubes can have different additives or coatings
Evacuated Collection Tubes
- The gauge number indicates the bore size: the larger the gauge number, the smaller
the needle bore. Needles are available for evacuated systems and for use with a syringe, single
draw or butterfly system.
Needles
use with the evacuated collection/tube system.
Tube Holder/Adapter
used if blood culture is to be drawn.
Povidone-iodine wipes/swabs
For application on the site from which the needle is withdrawn
Gauze sponges
protects the venipuncture site after collection.
Adhesive bandages/tape
can be made of latex, rubber, vinyl, etc; worn to protect the patient and the phlebotomist
Gloves
– may be used in place of the evacuated collection tube for special circumstances.
Syringes
ORDER OF DRAW:
- blood culture bottle(yellow-black top)
- coagulation tube (light blue)
- non-additive (red top)
- Serum separator tube (gold top/red-gray top)
- Sodium heparin (black-green top)
- PST/Plasma Separator Tube (light green top)
- EDTA (lavender top)
- ACDA (Acid Citrate Dextrose solution “A”)/ACDB (Acid Dextrose solution “B”)
- Oxalate/fluoride (light gray top)
Contains a gel separator and clot activator
SST/Serum Separator Tube (red-gray or gold top)