PRELAB 3.6 PRIMARY STRUCTURE Flashcards
Primary structures is the
AA sequence of polypeptides
linear sequence
Importance of primary structures
to understand mechanism of action of proteins
Primary sequence can be predicted base on the _______ which then determine __________ structures based on the
R chains or R groups
secondary and tertiary structures
sequence homologies w/ related proteins
Secondary structure is also called
substructure
Basic structure of the secondary structure of proteins
alpha helix
beta-pleated sheet
Alpha helix spiral structures are where the side chains extend _____
outwards
These secondary structure are also called _______ based on the _____ AA and forms ____, ______ and ___ within a polypeptide
localized arrangement
adjacent
coils, sheets, and loops
Alpha Helix, stabilizes _______ bonding between ______, and ______
hydrogen bonding
carbonyl oxygen group
amide hydrogen
Alpha helix is mostly found in
1) ______ (e.g., ____, ____)
2) _______ such as _____ (triple helix
globular proteins ( myoglobin, troponin C
structural such as collagen
It is when two or more polypetides chains lie side by side
Beta-pleated sheet
Beta-pleated sheet stabilized by ___ bond between ____ and _____ of adjacent chains
Give an example
[ DOUBLE CHECK KUNG AMINO OR AMIDE!!!!!]
hydrogen bond between NH group and carbonyl
silk fibroin from silk moths or silkworms
Primary structure refers to the _______ present in the _______
sequence of amino acids present in the polypeptide chains
Each component of the amino acid in a polypeptide is called _____ or _____
residue
moiety
1° structure of a protein starts from ____________ and ends with ___________
amino terminal (N) end
carboxyl-terminal (C) end
Importance of primary structure
1)
2)
3)
4)
1) structure prediction (from sequence homologies of related proteins)
2) Detect genetic diseases caused by abnormal AA seq
3) Understand molecular mechanism of action of proteins
4) Trace evolutionary paths (shared ancestry)
Two methods of AA sequence determination
end group analysis
gene sequencing method
End group analysis
1) method
2) aim
3) who
4) so basically it is …
edman degradation
determine the proteins C-terminal and N-terminal residue
Pehr Edman
sequencing of AA in a peptide
What is gene sequencing
What is the primary tool used for this
reveals the order of bases present in the genome of an organism
computer software/application that assembles enormous sequence data
