PRELAB 3.6 PRIMARY STRUCTURE

Question 1

Primary structures is the

Answer 1

AA sequence of polypeptides
linear sequence

Question 2

Importance of primary structures

Answer 2

to understand mechanism of action of proteins

Question 3

Primary sequence can be predicted base on the _______ which then determine __________ structures based on the

Answer 3

R chains or R groups

secondary and tertiary structures

sequence homologies w/ related proteins

Question 4

Secondary structure is also called

Answer 4

substructure

Question 5

Basic structure of the secondary structure of proteins

Answer 5

alpha helix
beta-pleated sheet

Question 5

Alpha helix spiral structures are where the side chains extend _____

Answer 5

outwards

Question 5

These secondary structure are also called _______ based on the _____ AA and forms ____, ______ and ___ within a polypeptide

Answer 5

localized arrangement
adjacent
coils, sheets, and loops

Question 6

Alpha Helix, stabilizes _______ bonding between ______, and ______

Answer 6

hydrogen bonding
carbonyl oxygen group
amide hydrogen

Question 7

Alpha helix is mostly found in

1) ______ (e.g., ____, ____)
2) _______ such as _____ (triple helix

Answer 7

globular proteins ( myoglobin, troponin C
structural such as collagen

Question 8

It is when two or more polypetides chains lie side by side

Answer 8

Beta-pleated sheet

Question 9

Beta-pleated sheet stabilized by ___ bond between ____ and _____ of adjacent chains

Give an example

Answer 9

[ DOUBLE CHECK KUNG AMINO OR AMIDE!!!!!]

hydrogen bond between NH group and carbonyl

silk fibroin from silk moths or silkworms

Question 10

Primary structure refers to the _______ present in the _______

Answer 10

sequence of amino acids present in the peptide bonds

Question 11

Each component of the amino acid in a polypeptide is called _____ or _____

Answer 11

residue
moiety

Question 12

1° structure of a protein starts from ____________ and ends with ___________

Answer 12

amino terminal (N) end
carboxyl-terminal (C) end

Question 13

Importance of primary structure
1)
2)
3)
4)

Answer 13

1) structure prediction (from sequence homologies of related proteins)
2) Detect genetic diseases caused by abnormal AA seq
3) Understand molecular mechanism of action of proteins
4) Trace evolutionary paths (shared ancestry)

Question 14

Two methods of AA sequence determination

Answer 14

end group analysis
gene sequencing method

Question 15

End group analysis
1) method
2) aim
3) who
4) so basically it is …

Answer 15

edman degradation
determine the proteins C-terminal and N-terminal residue
Pehr Edman
sequencing of AA in a peptide

Question 16

What is gene sequencing
What is the primary tool used for this

Answer 16

reveals the order of bases present in the genome of an organism

computer software/application that assembles enormous sequence data