PRELAB 3.3 HISTORY OF PROTEIN DISCOVERY

Question 1

Proteins adopt distinct _________ critical to their function
It is a result of interactions betwen _____

Answer 1

Distinct 3D conformations
AA constituents

Question 2

The first person who demonstrated the 3d conformation of protein

Answer 2

Christian Afinsen

Question 3

Explain the principles of Afinsen’s experiment
- process
- goal
- sample and results

Answer 3

• denaturation
  -disrupt then 3d structure via heat
• Native RNAse subjected to heat; non-covalent bond loosens and break

Question 4

RNase protein consists of ____ aa
It has _______,created by _____ which is a side chain since it has

Answer 4

124
4 disulfide bridges
Cysteine
Sulfur

Question 5

What condition must the denatured proteins be subjected to conform to its native state

Answer 5

Incubation with milder conditions

Question 6

How is the 3D structure of the protein viewed?

Answer 6

X-ray crystallography

Question 7

In the year _______
He ________ determined the 3D structure of ________

Answer 7

1958
John Kendrew
Myoglobin

Question 8

What did John Kendrew conclude with his observation?

Answer 8

Myoglobin is a simple protein consisting of 153 AA

Question 9

Myoglobin longer than _____ with only ______

Answer 9

ribonuclease
124 AA

Question 10

What is the structure of myoglobin?

Answer 10

• globular,
• polypeptide chain with compact form
• made up of long fibrous molecules