PRE LAB 4.3 KIRBY BAUER

Question 1

After inoculating the suspension on the MHA plates, impregnated with the antibiotic discs (controls) and incubate the plates for _____ hrs

Answer 1

24 hours

Question 2

Measuring of ZOIs uses _____
to view the plates better, place them above _______ surface

Answer 2

caliper or ruler
black, non-reflective surface

Question 3

the standard reference used for intepreting diameters is

Answer 3

clinical and laboratory standards institute (CLSI)

Question 4

If some microoganisms are not available in CLSI, ______ serves as a guideline as opposed to CLSI which uses ____________

Answer 4

minimum inhibitory concentration
zone diameter breakpoints

Question 5

Another institution aside from clinical and laboratory standards institute

Answer 5

EUCAST
European Committee on Antimicrobial Susceptibility testing

Question 6

difference between CLSI and EUCAST

Answer 6

Neisseria gonorrhoeae
Neisseria meningiditis
CLSI - zone diameter breakpts
EUCAST - MIC criteria (disk diffusion is not reliable)

Question 7

standard tool used in measuring diameter of zone of inhibition

Answer 7

calipers

Question 8

when overlap occurs, the zone diameter can be determine by the ____

Answer 8

radius zone

Question 9

interpretation of results using a template

suceptible

Answer 9

susceptible (outer zone) : outside the bigger black circle; outer zone

Question 10

interpretation of result using a template for
resistant

Answer 10

resistant (inner zone): no zone, within the white circle

Question 11

interpretation of result using a template for
intermediate

Answer 11

intermediate (black zone): lies on the black circle