PRE LAB 4.4 DILUTION METHOD

Question 1

MIC is used for testing extracts such as

Answer 1

plant extracts, metabolites

Question 2

MIC is done through dilution of antibiotics in either ___ or ____

Answer 2

agar or broth media

Question 3

MIC is determined by inoculating the organism from the sample into a series of tubes containing __________ of drug

Answer 3

progressive dilution

Question 4

3 steps of MIC in clinical trials

Answer 4

patient’s organism added into tubes with decreasing antibiotis
incubation overnight at 37 deg C
lowest conc. of dug that inhibits growth is the MIC

Question 5

difference between MIC and MBC

Answer 5

MIC- for slowed down microbial growth
MBC (Minimum bactericidal concentration) - lowest conc. that kills and eliminates bacteria

Question 6

this type of dilution adds drug in prepared agar and poured into plates

Answer 6

agar dilution

Question 7

advantages and disadvantages of agar dilution

Answer 7

advantages: many strains can be inoculated on each plate with antibiotic dilution

disadvantages: not easily automated, time consuming, requiring fresh reagents

Question 8

combines disk diffusion and dilution methods

Answer 8

epsilometer test

Question 9

e-test uses ____ which are impregnated with drug having exponential conc. scale

Answer 9

rectangular strips

Question 10

4 steps of E-test

Answer 10

apply E-test strip on an inoculated agar plate
immediate release of drug
24 hr incubation
symmetrical inhibition ellipse is produced

Question 11

how to know the MIC using the E-test

Answer 11

the conc. pointing at which the ellipse meets the strip is the MIC