PRE LAB 4.5 Agar well diffusion assay

Question 1

this method is preferred for some scientists because____

Answer 1

it gives better zone of inhibitions

Question 2

agar well difussion assay is widely used to evaluate the antimicrobial using ___________

Answer 2

plants or microbial extracts

Question 3

it is also used to test and see the _______ and ______ effect of different microorganisms

Answer 3

synergistic and antagonistic

Question 4

STEP 1: spread a volume of microbial inoculum over the entire agar surface

STEP 2: puncture a hole measuring ____________ with a sterile cork borer

STEP 3: dipense _______ amount of extract with the desired conc.

STEP 4: incubate the plates at optimal temp depending on the bacteria

Answer 4

6-8 mm
20-100 uL