PRE LAB 2: TYPES OF AUTOINDUCERS Flashcards
Autoinducer-1
intraspecies - same species
examples of species using AI-1
Serratia liquefaciens
Vibrio harveyi
Vibrio fischeri
Agrobacterium tumafaciens
Psuedomonas aeruginosa
Autoinducer-2
inter-species (for different types of receptors)
organisms of the same kind have the same receptor
QS1
one shape for all; universal signal/ligand
QS2
The signal and
receptor bind easily.
QS1
antibiotic resitance is caused by:
patient abuse- not following prescription
modern lifestyle - meats are injected with enhancers which can be transferred to humans upon consumption
how can quorum sensing be usedto fight antibiotic resistance
bacterial communication through quorum sensing can cause the formation of biofilm which adds protection to the pathogens. Controlling and interferring with bacterial communication by using the principles of quorum sensing we can prevent the formation of biofilm
Bioluminescene non pathogenic bacteria
V. fischeri
V. harveyi
Photobacterium spp.
Biofilm forming bacteria that are pathogenic
Pseudomonas aeruginosa PAO1
MRSA
E. coli 0157-H7
V. cholerae
How can biofilms affect the host infected
coordinate and release toxins to attacK the host
_______ forms biofilms in catheters and is the leading cause of hospital-acquired infections
Staphylococcus aureus
how can we use the princple of quorum sensing to prevent biofilm formation
disrupt the communication of autoinducers signals to prevent high cell density
How can the concept of quorum sensing be applied to control P. aeruginosa
peptide inducers such as AHL when it binds to the target bacteria can determine whether a chain will be experessed. there are peptide inducers that can disrupt the flow of transcription to prevent their communication. Controlling these genes might prevent the ability of these microbes to cause disease
