Practice Qs Flashcards
1
Q
Cell -
A
- time course
- electropheresis: intact fragment and the broken fragment
- negative control: KO or a different terminal
2
Q
fluorescent
A
- N and C at different emission peaks
- conjugate tag
3
Q
WGS
A
gene cloning and Sanger sequencing
4
Q
mutations
A
- not recognised by an enzyme
- truncated: can’t bind to protein, ribosome or promotor
- affinity: can’t bind
- steric hindrance
5
Q
auxin
A
relate everything back to ratios
6
Q
combination therapy
A
- fluctuating environment
- rapid adaptation due to more variation per generation ; more for NS to act on
7
Q
spot plating
A
don’t use if there’s a chance the mutation could have evolved before
8
Q
spot plating assumes
A
- the isolates grow at the same rate
- antibiotic sensitivity
- mutations acquired in the liquid pre-culture
9
Q
positive control
A
- purified enzyme
10
Q
artefacts on an electrophoresis
A
- other stuff recognised by the same Ab - is it functional?
11
Q
mutants to introduce in electrophoresis
A
- truncated mutants appear lower on the blot
- promotor, 5’ or ribosome binding mutants appear at the same weight but lower intensity
12
Q
what mutations cause AR
A
consider if they would be detrimental in other contexts
13
Q
Why has something evolved?
A
think about the environment - is it variable or stable
14
Q
frequency dependence
A
what would happen if you change concentrations?
