Autophagy Flashcards
1
Q
cytological EM
A
vesicles containing cytoplasm adjacent to vacuole
2
Q
cytological light microscopy
A
autophagic-cytoplasm containing bodies on vacuole
3
Q
TOR
A
- Ser/Thr kinase
- master regulator
- suppresses autophagy and regulates 26SP
- ATG13 phosphorylation
- no ATG1 kinase assembly
4
Q
Cytology
A
- ATG8 and 12 UBLs are PAS
- TOR inactivation: activated ATG1 kinase phosphorylates ATG4, ATG9
- small ATG9 vesicles localise to PIS
- ATG9 lipidates ATG8
- isolation membrane forms at or near ER
- thins (direct transfer of lipids via small vesicles by ATG8-PE), expands, curves (stabilised by actin sandwich, ATG8-PE binds ATG12-5-16), becomes spherical (requires COPII-ATG2 recruitment)
- pore closes (ATG9, ESCRT-mediated fission)
- autophagosome
- lysosome/vacuole translocation
- outer membrane fusion (v and t snares)
- hydrolase degradation and recycling
5
Q
IM
A
- phagophore
- small, flattened
6
Q
autophagosome
A
double-membrane bound
7
Q
ATG1 kinase
A
multiple complices assembles to form an autophagosomal assembly platform
8
Q
ATG9
A
- phosphorylated by ATG1 kinase
- multi-spanning membrane protein
9
Q
ATG4 protease
A
catalyses ATG8-PE delipidation
10
Q
In vitro liposomes
A
tethering, hemifusion and fusion
11
Q
microautophagy
A
direct invagination
12
Q
chaperone-mediated
A
Hsc70
13
Q
selective autophagy
A
- autophagy receptors bypass TOR
14
Q
ATG8-interacting-motif (AIM)
A
- short, 4 residue core sequences in hydrophobic patch
- ## phosphorylation activation
15
Q
Ub in selective autophagy
A
- labels cargo and bind to ATG8
