Eukaryotic DNA Replication Flashcards
1
Q
eukaryotic replication
A
- telomere-replication mechanism
- S-phase
2
Q
E. coli replication
A
4.6x10^6 bps in 40mins
3
Q
D. melanogaster replication
A
122x10^6bps in 4 mins
4
Q
Initiation
A
- ORC1-6 binds ori
- cdc6 binds ORC1-6
- loads 2x cdt1-Mcm2-7
- cdk phosphorylates Sld2/3
- GINS and pol-epsilon associate with Mcm2-7s
- ddk phosphorylates Mcms2-7
- activated: ssDNA, replication
5
Q
Mcm2-7
A
head-to-head double hexameters transiently opened to grasp dna
6
Q
when does licensing occur?
A
Late M/G1
7
Q
replicative polymerases
A
5’ -> 3’
8
Q
pol-alpha
A
2 subunits: rna fragment and pol1
9
Q
rna primers
A
facilitated by Ctf4 coupling pol-alpha with leading strand
10
Q
pol delta and epsilon
A
enhanced by PCNA
11
Q
PCNA
A
- proliferating cell nuclear antigen
- ring-like
- holds polymerases to template
12
Q
S. pombe ORC
A
ORC4 domain
13
Q
Mammal ORC
A
- epigenetic marks
- G4 structures
14
Q
origins
A
minimise transcription conflict
15
Q
geminin/SCF
A
triple-Ub tag on cdt1 on replication fork formation
