PCR Flashcards
1
Q
What is the function of PCR?
A
To amplify DNA using complementary primers for specific target sequences.
2
Q
What are primers?
A
Short strands of nucleotides which are complementary to specific target sequences at the two ends of the region of DNA to be amplified.
3
Q
What is the first stage of PCR?
A
The DNA is heated to between 92 and 98 degrees to separate the strands.
4
Q
What is the second stage of PCR?
A
The strands are cooled to between 50 and 65 degrees to allow primers to bind to target sequences.
5
Q
What is the third stage of PCR?
A
The DNA is heated to between 70 and 80 degrees for heat tolerant DNA polymerase to replicate the region of DNA.
6
Q
How can PCR be used?
A
To help solve crimes, settle paternity suits and diagnose genetic disorders.
