Neuroscience Techniques Flashcards

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0
Q

What is an example of a single cell?!

A

Cell lines or oocytes

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1
Q

What are the types of preparation for neuroscience techniques?!

A
Single cell
Brain/spinal cord slices
Larger in-vitro: brainstem/spinal cord
Intact nerve
In-vivo
Invertebrate
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2
Q

What is the advantages of spinal cord or brainstem slices?!

A

Local synaptic connections are intact

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3
Q

Which type of preparation is the most realistic?!

A

In-vivo (the whole animal)

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4
Q

What can intracellular recording techniques measure?!

A

Resting membrane potential
Action potential
Firing rate
Synaptic connections

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5
Q

What is the advantage of filling a neurone with a dye?!

A

Can be viewed under light microscopy so the neurone can be reconstructed

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6
Q

What do extra cellular recording techniques record?!

A

Voltage
Action potential firing rate
Field potentials (synaptic potentials)

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7
Q

What does the voltage clamp technique control?!

A

Voltage

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8
Q

In the voltage clamp experiment what does the recording electrode measure?!

A

The membrane potential

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9
Q

What does the voltage clamp amplifier inject?!

A

A current into the axon

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10
Q

What does the voltage clamp technique measure?!

A

Changes in current

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11
Q

How is the patch-clamp recording carried out?!

A

A glass electrode is placed against the membrane of a neurone and a suction force is applied forming a high resistance seal between the glass and the membrane

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12
Q

How large is the current flowing through a single channel?!

A

Miniscule - called a microscopic current

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13
Q

What type of techniques can be performed on a brain slice preparation?!

A

Current-clamp
Voltage-clamp
Patch-clamp

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14
Q

Give two examples of large cells.

A

Molluscan giant neurones

Xenopus oocytes

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15
Q

Why is the two electrode voltage clamp useful?!

A

For voltage clamping large cells

16
Q

When can two electrode voltage clamp not be used?!

A

With neurones in the mammalian brain due to their small size

17
Q

What do the two electrodes in two electide voltage clamp do?!

A

One records voltage and the other injects current

18
Q

What can xenopus oocytes be used for?!

A

Injected with exogenous mRNA sequences and they will synthesis the foreign protein in large quantities
Eg. Injecting the mRNA sequence of ion channels and they will express functional ion channels