Neuroanatomy Flashcards

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0
Q

What does formaldehyde do?!

A

Fixes proteins

Prevents auto lysis and decomposition

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1
Q

Why is brain tissue fixed with formaldehyde?!

A

Because it is very soft

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2
Q

What does the DAPI stain do?!

A

Detecte DNA in living and fixed neurones

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3
Q

What does Nissl stain do?!

A

Detects neurones and glia in the brain - stains the nuclei

Gives an idea of cytoarchitecture

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4
Q

What does the Golgi stain do?!

A

Detect neurones in the brain
Stains the cell body and neurites
Does not stain all of the neurites

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5
Q

What does nauta silver stain do?!

A

Detects degenerating axons

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6
Q

What is the limit of the light microscope?!

A

0.1 micrometers

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7
Q

What is the resolution of the electron microscope?!

A

0.1 nanometers

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8
Q

What type of microscopy is used for examining synapses etc?!

A

Electron - greater resolution

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9
Q

What is immunohistochemistry?!

A

Manufacturing antibodies to the candidate protein and by visualising these antibodies in sections of brain tissue

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10
Q

What type of labelling does immunohistochemistry allow?!

A

Discrete

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11
Q

What can immunohistochemistry be combined with?!

A

Fluorescent tags - allows the staining of multiple proteins

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12
Q

What is axoplasmic transport?!

A

Movement of material down the axon

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13
Q

What are microtubules used for?!

A

Used by neurones to transport substances from the cell body to the axon terminal

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14
Q

What is movement from the soma to axon terminals called?!

A

Anterograde transport

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15
Q

What is the movement from axon terminals to the soma called?!

A

Retrograde

16
Q

What does auto radiography do?!

A

Detects the position of radioactive axon terminals in tissue slices

17
Q

What is horseradish peroxide taken up by?!

A

Axon terminals and retrogradely transported to the cell body

18
Q

What is transneuronal tracing?!

A

More than one synapse