Myocardial Regeneration In Mammals, Amphibia And Fish

Question 1

Describe the transgenic zebrafish produceed by C. Belmonte

Answer 1

They expressed GFP in mature cardiomyoctes

Question 2

Describe the experiment to study regeneration in zebrafish?

Answer 2

The tip of the heart was cut away in transgenenic zebrafish, and a blood clot forms which keeps the zebrafish alive.
Regeneration begins immediately

Question 3

What type of regeneration occurs in zebrafish?

Answer 3

De-differentiation, proliferation, migration towards injured site and re-differentiation of cardiomyocytes - very little contribution from stem cells. This is similar to the regeneration in human liver.

Question 4

Describe regeneration of the heart in the newt

Answer 4

De-differentiation, proliferation and re-differentiation of cardiomyocytes

Question 5

What type of regeneration occurs after an MI in mice?

Answer 5

Limited regeneration via the local population of adult stem cells