Mosby's Sterilization

Question 1

1. ___ is the removal of ALL micro organisms including spores

Answer 1

sterilization

Question 2

__ eliminates MOST micro organisms but does not include the removal of bacterial SPORES and SOME VIRUSES and FUNGI

Answer 2

disinfection

Question 3

sodium hypochlorite is a __

Answer 3

surface disinfectant

Question 4

sodium hypochlorite destroys cells via

Answer 4

oxidation

Question 5

advantages of sodium hypochlorite

disadvantage

Answer 5

broad spectrum and inexpensive.

toxic to tissues (bleach)

Question 6

what is often used as an intracanal irrigant during root canal therapy

Answer 6

sodium hypochlorite

Question 7

___are disinfectants that can be applied to the skin

Answer 7

antiseptics

Question 8

1. Autoclave uses __ heat
2. mech of action
3. advantages
4. disadvantages

Answer 8

moist heat.
denature proteins.
short time and very effective and dependable
can damage heat sensitive items

Question 9

autoclave temp

Answer 9

wrapped instruments 121C/250F/15psi for 15 min.
unwrapped flash cycle: 134C/270F/30psi
for 3 min

Question 10

which sterilization method is non corrosive to metals

Answer 10

dry heat :
160C for 2 hours
171C for 1 hour.
disadvantage: longer time than moist heat and can damage heat sensitive instruments

2% glutaraldehyde

Question 11

what could you use for heat/moisture sensitive instruments

Answer 11

ethylene oxide gas 1-12 hours. alkylating agent that denatures nucleic acids and proteins (DNA)
or 2% glutaraldehyde

Question 12

disadvantages of ethylene oxide gas?

Answer 12

very toxic flammable and takes a long time

Question 13

Name the sterilization by the mode of action:

1. denatures nucleic acids and proteins (DNA)
2. denatures proteins
3. coagulation of proteins

Answer 13

1. 2% glutaraldehyde and ethylene oxide gas
2. autoclave moist heat
3. dry heat

Question 14

2% glutaraldehyde time required

Answer 14

12 hours

Question 15

advantages for 2% glutaraldehyde

Answer 15

non corrosive to metal and good for heat sensitive and moisture sensitive

Question 16

disadvantages of 2% glutaraldehyde

Answer 16

long time, very toxic and flammable (like ethylene gas)

Question 17

Antiseptics:

1. quaternary ammonium compounds
2. formaldehyde soaps and detergents
3. 2% tincture of iodine
4. alcohol (70% ethanol)

which is the most effective antiseptic

Answer 17

3. 2% tincture of iodine

disrupts cell membranes

Question 18

Antiseptics:

1. quaternary ammonium compounds
2. formaldehyde soaps and detergents
3. 2% tincture of iodine
4. alcohol

which one: denatures proteins 
low toxicity 
effective against most vegetative bacteria fungi and viruses
limited contact time bc evaporates fast
decreased action

Answer 18

alcohol (70% ethanol)