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OCR (A) A Level Biology - Module 2 - Chapter 2 > More microscopy - 2.3 (2) > Flashcards

More microscopy - 2.3 (2) Flashcards

Module 2, Chapter 2, 2.3, Page 19 & 20

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1
Q

What is inside of an electron microscope?

A

a vaccuum to ensure the electron beams travel in straight lines

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2
Q

Samples need to be…

A

processed in a specific way

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3
Q

Specimen preparation: fixation using…

A

chemicals or freezing

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4
Q

Specimen preparation: staining with…

A

heavy metal

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5
Q

Specimen preparation: dehydration with…

A

solvents

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6
Q

What will samples for a TEM be set in?

A

resin

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7
Q

Will samples for a TEM possibly be stained again?

A

yes

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8
Q

What will samples for SEM be fractured to expose?

A

the inside

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9
Q

What will the samples for a SEM be coated with?

A

heavy metals

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10
Q

What is inexpensive to buy and operate?

A

light microscope

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11
Q

What type of microscope is small and portable?

A

light microscope

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12
Q

Do light microscopes have a simple sample preparation?

A

yes

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13
Q

What type of microscope does not usually lead to distortion in sample preparation?

A

light microscope

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14
Q

Is a vacuum required in light microscopy?

A

no

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15
Q

Can natural colour of samples be seen in light microscopy?

A

yes

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16
Q

What magnification can light microscopes go up to?

A

x2000

17
Q

What resolving power do light microscopes have?

A

200nm

18
Q

What kind of specimens can be used in light microscopes?

A

living or dead

19
Q

Electron microscopes are expensive to…

A

buy and operate

20
Q

Electron microscopes are large and…

A

need to be installed

21
Q

What kind of microscope has a complex sample preparation?

A

electron microscope

22
Q

Which microscope has a sample preparation that often distorts materials?

A

electron microscope

23
Q

Is vacuum required in electron microscopes?

A

yes

24
Q

What colour images are produced in electron microscopes?

A

black and white (but can be coloured digitally)

25
Q

What magnification can electron microscopes have up to?

A

x500 000

26
Q

What resolving power do transmission electron microscopes have?

A

0.5nm

27
Q

What resolving power do scanning electron microscopes have?

A

3-10nm

28
Q

Specimens are…

A

dead in electron microscopes

OCR (A) A Level Biology - Module 2 - Chapter 2 (24 decks)

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