More microscopy - 2.3 (2) Flashcards

Module 2, Chapter 2, 2.3, Page 19 & 20

1
Q

What is inside of an electron microscope?

A

a vaccuum to ensure the electron beams travel in straight lines

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2
Q

Samples need to be…

A

processed in a specific way

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3
Q

Specimen preparation: fixation using…

A

chemicals or freezing

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4
Q

Specimen preparation: staining with…

A

heavy metal

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5
Q

Specimen preparation: dehydration with…

A

solvents

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6
Q

What will samples for a TEM be set in?

A

resin

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7
Q

Will samples for a TEM possibly be stained again?

A

yes

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8
Q

What will samples for SEM be fractured to expose?

A

the inside

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9
Q

What will the samples for a SEM be coated with?

A

heavy metals

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10
Q

What is inexpensive to buy and operate?

A

light microscope

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11
Q

What type of microscope is small and portable?

A

light microscope

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12
Q

Do light microscopes have a simple sample preparation?

A

yes

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13
Q

What type of microscope does not usually lead to distortion in sample preparation?

A

light microscope

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14
Q

Is a vacuum required in light microscopy?

A

no

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15
Q

Can natural colour of samples be seen in light microscopy?

A

yes

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16
Q

What magnification can light microscopes go up to?

17
Q

What resolving power do light microscopes have?

18
Q

What kind of specimens can be used in light microscopes?

A

living or dead

19
Q

Electron microscopes are expensive to…

A

buy and operate

20
Q

Electron microscopes are large and…

A

need to be installed

21
Q

What kind of microscope has a complex sample preparation?

A

electron microscope

22
Q

Which microscope has a sample preparation that often distorts materials?

A

electron microscope

23
Q

Is vacuum required in electron microscopes?

24
Q

What colour images are produced in electron microscopes?

A

black and white (but can be coloured digitally)

25
What magnification can electron microscopes have up to?
x500 000
26
What resolving power do transmission electron microscopes have?
0.5nm
27
What resolving power do scanning electron microscopes have?
3-10nm
28
Specimens are...
dead in electron microscopes