Magnification and calibration - 2.2 (1) Flashcards

Module 2, Chapter 2, 2.2, Page 15

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1
Q

What is magnification?

A

how many times larger the image is than the actual size of the object being viewed

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2
Q

What do the interchangeable objective lenses on a compound light microscope allow a user to adjust?

A

the magnification

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3
Q

What cannot be done just by simply magnifying an object?

A

increasing the amount of detail that can be seen

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4
Q

What also needs to be increased apart from magnification?

A

the resolution

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5
Q

What determines the amount of detail that can be seen?

A

the resolution of a microscope

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6
Q

The higher the resolution…

A

the more details are visible

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7
Q

What is resolution?

A

it its the ability to see individual objects as seperate entities

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8
Q

What is resolution limited by?

A

the diffraction of light as it passes through samples (and lenses)

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9
Q

What is diffraction?

A

it is the tendency of light waves to spread as they pass close to physical structures that are present in the specimens

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10
Q

How are the structures in the specimens arranged?

A

very close to eachother

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11
Q

What can happen as an outcome when the light is reflected from individual structures in the specimen?

A

the light can overlap due to diffraction

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12
Q

In optical microscopy, structures that are closer than half the wavelength of light cannot be…

A

seen seperately

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13
Q

How can resolution be increased?

A

by using beams of electrons which have a wavelength thousands of times shorter than light

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14
Q

What is still diffracted?

A

electron beams

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15
Q

What is meant by the shorter wavelength through electron beams?

A

individual beams can be much closer before they overlap

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16
Q

Objects which are much smaller and closer together…

A

can be seen separately without diffraction blurring the image

17
Q

What is the magnification formula?

A

magnification = size of image / actual size of object

18
Q

The size of the image refers to the…

A

length of the image as measured, for example with a ruler

19
Q

What do you need to change to that of the actual size of the object?

A

the units of measurement

20
Q

Can the magnification formula be rearranged to find any of the unknowns?

A

yes, through the three-part formula triangle